Identification of Novel Quantitative Trait Loci Linked to Crown Rot Resistance in Spring Wheat
Abstract
:1. Introduction
2.Results
2.1. Fusarium culmorum Disease Assessment
2.2. Genotyping with Diversity Arrays Technology (DArT)Markers
2.3. Structure Analysis
2.4. Linkage Disequilibrium
2.5. Marker-Trait Associations for F. culmorum Resistance
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Inoculum Preparation
4.3. Fusarium culmorum Disease Screening
4.3.1. Growth Room
4.3.2. Greenhouse
4.3.3. Field Conditions
4.4. Disease Assessment and Data Analysis
4.5. Genotyping with DArT Markers
4.6. Population Structure Analysis
4.7. Linkage Disequilibrium and GWAS
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Cross Name | CID | Adult-Plant Resistance | Seedling Resistance | |||
---|---|---|---|---|---|---|
GHSS | GHCS | FCS | GRCS | RR | ||
BABAX/LR42//BABAX/3/BABAX/LR42//BABAX/4/T.DICOCCON PI94625/AE.SQUARROSA (372)//3*PASTOR/5/T.DICOCCON PI94625/AE.SQUARROSA (372)//3*PASTOR | 6,000,229 | 2.2 | 2.5 | 2.2 | 2.5 | A-S |
BABAX/LR42//BABAX/3/BABAX/LR42//BABAX/4/T.DICOCCON PI94625/AE.SQUARROSA (372)//3*PASTOR/5/T.DICOCCON PI94625/AE.SQUARROSA (372)//3*PASTOR | 6,000,238 | 1.5 | 2.2 | 2.7 | 2.2 | S |
FRET2*2/4/SNI/TRAP#1/3/KAUZ*2/TRAP//KAUZ/5/ONIX | 6,000,365 | 1.5 | 2.5 | 3 | 1.8 | S |
ACHTAR/4/MILAN/KAUZ//PRINIA/3/BAV92 | 6,000,537 | 2.2 | 2.5 | 2.5 | 2.3 | A-S |
SOKOLL*2/ROLF07 | 6,000,973 | 1.5 | 2.5 | 2.3 | 3.2 | A |
GK ARON/AG SECO 7846//2180/4/2*MILAN/KAUZ//PRINIA/3/BAV92 | 6,001,016 | 2.5 | 3.5 | 3.2 | 1.8 | S |
SOKOLL//FRTL/2*PIFED | 6,001,172 | 2.2 | 2.8 | 2.5 | 2.3 | A-S |
ROLF07/3/T.DICOCCON PI94625/AE.SQUARROSA (372)//3*PASTOR | 6,001,240 | 2.2 | 2.8 | 3 | 2.3 | S |
CUNNINGHAM/4/SNI/TRAP#1/3/KAUZ*2/TRAP//KAUZ | 6,001,457 | 2.2 | 2.8 | 3 | 1.8 | S |
SOKOLL*2/4/CHEN/AEGILOPS SQUARROSA (TAUS)//FCT/3/STAR | 6,001,643 | 2.5 | 3.5 | 2.7 | 2.3 | S |
SERI*3//RL6010/4*YR/3/PASTOR/4/BAV92/5/MONARCA F2007/6/PVN//CAR422/ANA/5/BOW/CROW//BUC/PVN/3/YR/4/TRAP#1 | 6,000,104 | 2.2 | 2.3 | 2.5 | 2.8 | A |
CNO79//PF70354/MUS/3/PASTOR/4/BAV92/5/FRET2/KUKUNA//FRET2/6/MILAN/KAUZ//PRINIA/3/BAV92 | 6,000,615 | 1.5 | 2.8 | 3.5 | 2.3 | S |
2–49 | 1.3 | 2.3 | 2.5 | 2.5 | MR | |
Altay | 1.2 | 2.5 | 2.3 | 2.4 | MR | |
Sunco | 1.5 | 2.5 | 2.3 | 2.6 | MR | |
Seri | 2.2 | 3.2 | 3.5 | 4 | MS | |
Kiziltan | 2.5 | 3.5 | 4 | 4 | HS | |
Kutluk | 2.5 | 3.5 | 4 | 4 | HS | |
Wylie | 2.3 | 3.3 | 3.4 | 3.5 | S | |
Janz | 2.5 | 3.5 | 3.7 | 4 | HS | |
LSD | 0.5 | 0.53 | 1.04 | 1.08 | ||
Probability | <0.001 | <0.001 | <0.001 | <0.001 |
GHCS | FCR | GRCS | GHSS | |
---|---|---|---|---|
GHCS | 1.00000 | 0.10153 | 0.01128 | 0.50728 |
FCR | 0.2388 | 1.00000 | 0.31369 | 0.14685 |
GRCS | 0.89671 | 0.00010 | 1.00000 | 0.13237 |
GHSS | 0.00000 | 0.08574 | 0.12258 | 1.00000 |
Trait | Marker | CHR | POS (cM) | p-Value | FDR Adjusted p-Values | r2 (%) |
---|---|---|---|---|---|---|
FCS (GLM) | wPt-2193 | 3B | 39.1 | 6.56 × 10−5 | 0.01232 | 11.4 |
FCS (GLM) | wPt-2766 | 3B | 39.1 | 6.56 × 10−5 | 0.01232 | 11.4 |
GHSS (MLM) | wPt-669517 | 2D | 95.7 | 3.24 × 10−5 | 0.01997 | 11.6 |
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Erginbas-Orakci, G.; Sehgal, D.; Sohail, Q.; Ogbonnaya, F.; Dreisigacker, S.; Pariyar, S.R.; Dababat, A.A. Identification of Novel Quantitative Trait Loci Linked to Crown Rot Resistance in Spring Wheat. Int. J. Mol. Sci. 2018, 19, 2666. https://doi.org/10.3390/ijms19092666
Erginbas-Orakci G, Sehgal D, Sohail Q, Ogbonnaya F, Dreisigacker S, Pariyar SR, Dababat AA. Identification of Novel Quantitative Trait Loci Linked to Crown Rot Resistance in Spring Wheat. International Journal of Molecular Sciences. 2018; 19(9):2666. https://doi.org/10.3390/ijms19092666
Chicago/Turabian StyleErginbas-Orakci, Gul, Deepmala Sehgal, Quahir Sohail, Francis Ogbonnaya, Susanne Dreisigacker, Shree R. Pariyar, and Abdelfattah A. Dababat. 2018. "Identification of Novel Quantitative Trait Loci Linked to Crown Rot Resistance in Spring Wheat" International Journal of Molecular Sciences 19, no. 9: 2666. https://doi.org/10.3390/ijms19092666