Next Article in Journal
Innovative Approach of Non-Thermal Plasma Application for Improving the Growth Rate in Chickens
Previous Article in Journal
Contribution of STAT3 to Inflammatory and Fibrotic Diseases and Prospects for its Targeting for Treatment
Previous Article in Special Issue
Nucleolar GTP-Binding Protein 1-2 (NOG1-2) Interacts with Jasmonate-ZIMDomain Protein 9 (JAZ9) to Regulate Stomatal Aperture during Plant Immunity
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2018, 19(8), 2300; https://doi.org/10.3390/ijms19082300

Transcriptomic Analyses of Root Restriction Effects on Phytohormone Content and Signal Transduction during Grape Berry Development and Ripening

1
Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zijingang Campus, Zhejiang University, Hangzhou 310058, China
2
College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China
3
School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
4
Wujiang Research Institute of Grape, Jinhua 321017, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 12 July 2018 / Revised: 26 July 2018 / Accepted: 31 July 2018 / Published: 6 August 2018
View Full-Text   |   Download PDF [1623 KB, uploaded 6 August 2018]   |  

Abstract

Phytohormones strongly influence growth, development and nutritional quality of agricultural products by modulating molecular and biochemical changes. The purpose of the present study was to investigate the influence of root restriction (RR) treatment on the dynamic changes of main phytohormones during the berry development and ripening of “Summer Black” early ripening seedless grape (Vitis vinifera × V. labrusca), and to analyze the changes in the biosynthesis and signal transduction pathways of phytohormones by transcriptomics. Enzyme-linked immunosorbent assay (ELISA) and Ultra Performance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS) were used to quantify the phytohormone levels, and RNA-Seq was used to analyze the transcript abundance. The results showed that 23 transcripts involved in the phytohormone biosynthesis and 34 transcripts involved in the signal transduction pathways were significantly changed by RR treatment. RR also increased abscisic acid, brassinosteroid, ethylene, jasmonic acid and salicylic acid levels, while decreasing auxin, cytokinin, and gibberellin contents. The results of the present study suggest that RR treatment can accelerate the grape ripening process, and specific candidate genes were identified for further functional analysis. View Full-Text
Keywords: root restriction; grape development; phytohormones; RNA-Seq root restriction; grape development; phytohormones; RNA-Seq
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Leng, F.; Cao, J.; Wang, S.; Jiang, L.; Li, X.; Sun, C. Transcriptomic Analyses of Root Restriction Effects on Phytohormone Content and Signal Transduction during Grape Berry Development and Ripening. Int. J. Mol. Sci. 2018, 19, 2300.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top