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Int. J. Mol. Sci. 2018, 19(7), 1969; https://doi.org/10.3390/ijms19071969

Identification and Functional Analysis of the psaD Promoter of Chlorella vulgaris Using Heterologous Model Strains

1
Department of Life Science, College of Natural Sciences, Hanyang University, Seoul 04763, Korea
2
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
*
Author to whom correspondence should be addressed.
Received: 18 May 2018 / Revised: 22 June 2018 / Accepted: 5 July 2018 / Published: 6 July 2018
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Chlorella has great potential as a bio-factory for production of value-added compounds. To produce the desired chemicals more efficiently in Chlorella, genetic tools for modification of Chlorella need to be developed, especially an endogenous promoter. In this study, the promoter of photosystem I protein D (psaD) from Chlorella vulgaris UTEX395 was identified. Computational analysis revealed the presence of several putative cis-acting elements, including a potential core element, and light-responsive or stress-responsive elements. Gene expression analysis in heterologous expression system in Chlamydomonasreinhardtii and Nicotianabenthamiana showed that CvpsaD promoter can be used to drive the expression of genes. Functional analysis of this promoter suggested that the initiator element (Inr) is important for its function (i.e., TATA-less promoter) and that an additional factor (e.g., downstream of the transcriptional start site) might be needed for light response. We have shown that the CvpsaD promoter is functional, but not sufficiently strong, both in microalgae and higher plant. View Full-Text
Keywords: Chlorella vulgaris; native promoter; photosystem I protein D (psaD); heterologous expression Chlorella vulgaris; native promoter; photosystem I protein D (psaD); heterologous expression
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Kim, J.; Liu, L.; Hu, Z.; Jin, E. Identification and Functional Analysis of the psaD Promoter of Chlorella vulgaris Using Heterologous Model Strains. Int. J. Mol. Sci. 2018, 19, 1969.

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