Insights into the Origin of Distinct Medin Fibril Morphologies Induced by Incubation Conditions and Seeding
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Expression and Purification of Medin
4.2. Incubation of Medin
4.3. Thioflavin T Fluorescence
4.4. NMR
4.5. Intrinsic Fluorescence
4.6. Atomic Force Microscopy
4.7. Cell Viability
4.8. Circular Dichroism
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
Abbreviations
AFM | Atomic Force Microscopy |
CCK-8 | Cell Counting Kit-8 |
CD | Circular Dichroism |
GdmC | Guanidine hydrochloride |
IF | Intrinsic Fluorescence |
NMR | Nuclear Magnetic Resonance |
ThT | Thioflavin T |
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Secondary Structure | Time (h) | ||||||
---|---|---|---|---|---|---|---|
0 | 8 | 16 | 24 | 32 | 40 | 48 | |
Helix | 18 | 23 | 0 | 0 | 0 | 0 | 0 |
Sheet | 8 | 7 | 58 | 59 | 60 | 63 | 67 |
Turn | 27 | 27 | 20 | 19 | 18 | 19 | 7 |
Unordered | 47 | 42 | 22 | 21 | 22 | 18 | 25 |
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Davies, H.A.; Lee, C.F.; Miller, L.; Liu, L.-N.; Madine, J. Insights into the Origin of Distinct Medin Fibril Morphologies Induced by Incubation Conditions and Seeding. Int. J. Mol. Sci. 2018, 19, 1357. https://doi.org/10.3390/ijms19051357
Davies HA, Lee CF, Miller L, Liu L-N, Madine J. Insights into the Origin of Distinct Medin Fibril Morphologies Induced by Incubation Conditions and Seeding. International Journal of Molecular Sciences. 2018; 19(5):1357. https://doi.org/10.3390/ijms19051357
Chicago/Turabian StyleDavies, Hannah A., Chiu Fan Lee, Leanne Miller, Lu-Ning Liu, and Jillian Madine. 2018. "Insights into the Origin of Distinct Medin Fibril Morphologies Induced by Incubation Conditions and Seeding" International Journal of Molecular Sciences 19, no. 5: 1357. https://doi.org/10.3390/ijms19051357