Hinokitiol Inhibits Migration of A549 Lung Cancer Cells via Suppression of MMPs and Induction of Antioxidant Enzymes and Apoptosis
Abstract
:1. Introduction
2. Results
2.1. Cytotoxic Effect of Hinokitiol in A549 Cells
2.2. Hinokitiol Inhibits the Migration of A549 Cells
2.3. Effects of Hinokitiol on Caspase Signaling Pathway Activation
2.4. Hinokitiol Inhibits MMP-9 and MMP-2 Expression
2.5. Hinokitiol Enhances Antioxidants Enzymes in A549 Cells
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Cell Cultivation and Hinokitiol Treatment
4.3. Cell Viability
4.4. Wound Healing Migration Assay
4.5. Western Blotting
4.6. Non-Denaturing Polyacrylamide Gel Electrophoresis (Native PAGE)
4.6.1. CAT Activity Staining
4.6.2. SOD Activity Staining
4.7. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Jayakumar, T.; Liu, C.-H.; Wu, G.-Y.; Lee, T.-Y.; Manubolu, M.; Hsieh, C.-Y.; Yang, C.-H.; Sheu, J.-R. Hinokitiol Inhibits Migration of A549 Lung Cancer Cells via Suppression of MMPs and Induction of Antioxidant Enzymes and Apoptosis. Int. J. Mol. Sci. 2018, 19, 939. https://doi.org/10.3390/ijms19040939
Jayakumar T, Liu C-H, Wu G-Y, Lee T-Y, Manubolu M, Hsieh C-Y, Yang C-H, Sheu J-R. Hinokitiol Inhibits Migration of A549 Lung Cancer Cells via Suppression of MMPs and Induction of Antioxidant Enzymes and Apoptosis. International Journal of Molecular Sciences. 2018; 19(4):939. https://doi.org/10.3390/ijms19040939
Chicago/Turabian StyleJayakumar, Thanasekaran, Chao-Hong Liu, Guan-Yi Wu, Tzu-Yin Lee, Manjunath Manubolu, Cheng-Ying Hsieh, Chih-Hao Yang, and Joen-Rong Sheu. 2018. "Hinokitiol Inhibits Migration of A549 Lung Cancer Cells via Suppression of MMPs and Induction of Antioxidant Enzymes and Apoptosis" International Journal of Molecular Sciences 19, no. 4: 939. https://doi.org/10.3390/ijms19040939