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Int. J. Mol. Sci. 2018, 19(3), 675; https://doi.org/10.3390/ijms19030675

Key Anti-Fibrosis Associated Long Noncoding RNAs Identified in Human Hepatic Stellate Cell via Transcriptome Sequencing Analysis

1
The Research Center for Integrative Medicine, School of Fundamental Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
2
School of Chinese Materia Medical, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
*
Author to whom correspondence should be addressed.
Received: 11 December 2017 / Revised: 23 January 2018 / Accepted: 24 February 2018 / Published: 27 February 2018
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Hepatic fibrosis is the main pathological basis for chronic cirrhosis, and activated hepatic stellate cells (HSCs) are the primary cells involved in liver fibrosis. Our study analyzed anti-fibrosis long noncoding RNAs (lncRNAs) in activated human HSCs (hHSCs). We performed RNA sequencing (RNA-seq) and bioinformatics analysis to determine whether lncRNA expression profile changes between hHSCs activation and quiescence. Eight differentially expressed (DE) lncRNAs and three pairs of co-expression lncRNAs-mRNAs were verified by quantitative Real-Time Polymerase Chain Reaction (qRT-PCR). A total of 34146 DE lncRNAs were identified in this study. Via gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, we found several DE lncRNAs regulated hHSC activation by participating in DNA bending/packaging complex, growth factor binding and the Hippo signaling pathway (p < 0.05). With lncRNA–mRNA co-expression analysis, three lncRNAs were identified to be associated with connective tissue growth factor (CTGF), fibroblast growth factor 2 (FGF2) and netrin-4 (NTN4). The quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) results of the eight DE lncRNAs and three pairs of co-expression lncRNAs–mRNAs were consistent with the RNA-seq data and previous reports. Several lncRNAs may serve as potential targets to reverse the progression of liver fibrosis. This study provides a first insight into lncRNA expression profile changes associated with activated human HSCs. View Full-Text
Keywords: RNA-sequencing; lncRNA; hepatic fibrosis; human hepatic stellate cell; fibroblast growth factor 2 (FGF2); netrin-4 (NTN4) RNA-sequencing; lncRNA; hepatic fibrosis; human hepatic stellate cell; fibroblast growth factor 2 (FGF2); netrin-4 (NTN4)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Li, X.-Q.; Ren, Z.-X.; Li, K.; Huang, J.-J.; Huang, Z.-T.; Zhou, T.-R.; Cao, H.-Y.; Zhang, F.-X.; Tan, B. Key Anti-Fibrosis Associated Long Noncoding RNAs Identified in Human Hepatic Stellate Cell via Transcriptome Sequencing Analysis. Int. J. Mol. Sci. 2018, 19, 675.

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