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Int. J. Mol. Sci. 2018, 19(2), 396; https://doi.org/10.3390/ijms19020396

Comparative Transcriptome Analyses Uncover Key Candidate Genes Mediating Flight Capacity in Bactrocera dorsalis (Hendel) and Bactrocera correcta (Bezzi) (Diptera: Tephritidae)

Key Laboratory of Ministry of Agriculture for Monitoring and Green Management of Crop Pests, Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China
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Received: 5 December 2017 / Revised: 8 January 2018 / Accepted: 22 January 2018 / Published: 30 January 2018
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Flight capacity is important for invasive pests during entry, establishment and spreading. Both Bactrocera dorsalis Hendel and Bactrocera correcta Bezzi are invasive fruit flies but their flight capacities differ. Here, a tethered flight mill test demonstrated that B. dorsalis exhibits a greater flight capacity than B. correcta. RNA-Seq was used to determine the transcriptomic differences associated with the flight capacity of two Bactrocera species. Transcriptome data showed that 6392 unigenes were differentially expressed between the two species in the larval stage, whereas in the adult stage, 4104 differentially expressed genes (DEGs) were identified in females, and 3445 DEGs were observed in males. The flight capacity appeared to be correlated with changes in the transcriptional levels of genes involved in wing formation, flight muscle structure, energy metabolism, and hormonal control. Using RNA interference (RNAi) to verify the function of one DEG, the epidermal growth factor receptor (EGFR), we confirmed the role of this gene in regulating wing development, and thereby flight capacity, in both species. This work reveals the flight mechanism of fruit flies and provides insight into fundamental transcriptomics for further studies on the flight performance of insects. View Full-Text
Keywords: Bactrocera dorsalis Hendel; Bactrocera correcta Bezzi; flight capacity; transcriptome; RNA interference; EGFR; energy metabolism; juvenile hormone Bactrocera dorsalis Hendel; Bactrocera correcta Bezzi; flight capacity; transcriptome; RNA interference; EGFR; energy metabolism; juvenile hormone
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Guo, S.; Zhao, Z.; Liu, L.; Li, Z.; Shen, J. Comparative Transcriptome Analyses Uncover Key Candidate Genes Mediating Flight Capacity in Bactrocera dorsalis (Hendel) and Bactrocera correcta (Bezzi) (Diptera: Tephritidae). Int. J. Mol. Sci. 2018, 19, 396.

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