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Int. J. Mol. Sci. 2017, 18(9), 1862; doi:10.3390/ijms18091862

Human Meibum Age, Lipid–Lipid Interactions and Lipid Saturation in Meibum from Infants

1
Department of Ophthalmology and Visual Sciences, University of Louisville, Louisville, KY 40202, USA
2
Department of Chemistry, University of Louisville, Louisville, KY 40292, USA
3
Division of Ophthalmology, Children’s Hospital of Orange County, Orange, CA 92868, USA
4
Model Membranes Lab, Department of Biochemistry, Faculty of Biology, St. Kliment Ohridski University of Sofia, Sofia 1164, Bulgaria
5
Department of Pediatrics, University of Louisville, Louisville, KY 40202, USA
*
Author to whom correspondence should be addressed.
Received: 28 April 2017 / Revised: 25 July 2017 / Accepted: 17 August 2017 / Published: 28 August 2017
(This article belongs to the Special Issue Dry Eye and Ocular Surface Disorders)
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Abstract

Tear stability decreases with increasing age and the same signs of instability are exacerbated with dry eye. Meibum lipid compositional changes with age provide insights into the biomolecules responsible for tear film instability. Meibum was collected from 69 normal donors ranging in age from 0.6 to 68 years of age. Infrared spectroscopy was used to measure meibum lipid phase transition parameters. Nuclear magnetic resonance spectroscopy was used to measure lipid saturation. Increasing human meibum lipid hydrocarbon chain unsaturation with age was related to a decrease in hydrocarbon chain order, cooperativity, and in the phase transition temperature. The change in these parameters was most dramatic between 1 and 20 years of age. Meibum was catalytically saturated to determine the effect of saturation on meibum lipid phase transition parameters. Hydrocarbon chain saturation was directly related to lipid order, phase transition temperature, cooperativity, changes in enthalpy and entropy, and could account for the changes in the lipid phase transition parameters observed with age. Unsaturation could contribute to decreased tear film stability with age. View Full-Text
Keywords: age; dry eye; FTIR; lipids; meibum; NMR; spectroscopy age; dry eye; FTIR; lipids; meibum; NMR; spectroscopy
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Sledge, S.; Henry, C.; Borchman, D.; Yappert, M.C.; Bhola, R.; Ramasubramanian, A.; Blackburn, R.; Austin, J.; Massey, K.; Sayied, S.; Williams, A.; Georgiev, G.; Schikler, K.N. Human Meibum Age, Lipid–Lipid Interactions and Lipid Saturation in Meibum from Infants. Int. J. Mol. Sci. 2017, 18, 1862.

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