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Int. J. Mol. Sci. 2017, 18(7), 1424; doi:10.3390/ijms18071424

Chrysin Attenuates VCAM-1 Expression and Monocyte Adhesion in Lipopolysaccharide-Stimulated Brain Endothelial Cells by Preventing NF-κB Signaling

1,†
,
1,†,‡
and
1,2,*
1
College of Pharmacy, Ajou University, Suwon 16499, Korea
2
Research Institute of Pharmaceutical Sciences and Technology, Ajou University, Suwon 16499, Korea
These authors contributed equally to this work.
Present address: Il-Yang Pharm, Yongin 17096, Korea.
*
Author to whom correspondence should be addressed.
Received: 30 May 2017 / Revised: 22 June 2017 / Accepted: 27 June 2017 / Published: 3 July 2017
(This article belongs to the Special Issue Blood–Brain Barrier in CNS Injury and Repair)
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Abstract

Adhesion of leukocytes to endothelial cells plays an important role in neuroinflammation. Therefore, suppression of the expression of adhesion molecules in brain endothelial cells may inhibit neuroinflammation. Chrysin (5,7-dihydroxyflavone) is a flavonoid component of propolis, blue passion flowers, and fruits. In the present study, we examined the effects of chrysin on lipopolysaccharide (LPS)-induced expression of vascular cell adhesion molecule-1 (VCAM-1) in mouse cerebral vascular endothelial (bEnd.3) cells. In bEnd.3 cells, LPS increased mRNA expression of VCAM-1 in a time-dependent manner, and chrysin significantly decreased LPS-induced mRNA expression of VCAM-1. Chrysin also reduced VCAM-1 protein expression in a concentration-dependent manner. Furthermore, chrysin blocked adhesion of monocytes to bEnd.3 cells exposed to LPS. Nuclear factor-κB (NF-κB), p38 mitogen-activated protein kinase (MAPK), and c-Jun N-terminal kinase, which are all activated by LPS, were significantly inhibited by chrysin. These results indicate that chrysin inhibits the expression of VCAM-1 in brain endothelial cells by inhibiting NF-κB translocation and MAPK signaling, resulting in the attenuation of leukocyte adhesion to endothelial cells. The anti-inflammatory effects of chrysin suggest a possible therapeutic application of this agent to neurodegenerative diseases, such as multiple sclerosis, septic encephalopathy, and allergic encephalomyelitis. View Full-Text
Keywords: chrysin; neuroinflammation; blood-brain barrier; vascular cell adhesion molecule-1 (VCAM-1); lipopolysaccharide; monocyte adhesion; brain endothelial cell chrysin; neuroinflammation; blood-brain barrier; vascular cell adhesion molecule-1 (VCAM-1); lipopolysaccharide; monocyte adhesion; brain endothelial cell
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MDPI and ACS Style

Lee, B.K.; Lee, W.J.; Jung, Y.-S. Chrysin Attenuates VCAM-1 Expression and Monocyte Adhesion in Lipopolysaccharide-Stimulated Brain Endothelial Cells by Preventing NF-κB Signaling. Int. J. Mol. Sci. 2017, 18, 1424.

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