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Int. J. Mol. Sci. 2017, 18(6), 1299; doi:10.3390/ijms18061299

Gene Expression Profiling Reveals Potential Players of Left-Right Asymmetry in Female Chicken Gonads

1
State key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China
2
College of Life Sciences, Peking University, Beijing 100871, China
3
Annoroad Gene Technology Co., Ltd., Beijing 100176, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 4 May 2017 / Revised: 9 June 2017 / Accepted: 9 June 2017 / Published: 20 June 2017
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Most female birds develop only a left ovary, whereas males develop bilateral testes. The mechanism underlying this process is still not completely understood. Here, we provide a comprehensive transcriptional analysis of female chicken gonads and identify novel candidate side-biased genes. RNA-Seq analysis was carried out on total RNA harvested from the left and right gonads on embryonic day 6 (E6), E12, and post-hatching day 1 (D1). By comparing the gene expression profiles between the left and right gonads, 347 differentially expressed genes (DEGs) were obtained on E6, 3730 were obtained on E12, and 2787 were obtained on D1. Side-specific genes were primarily derived from the autosome rather than the sex chromosome. Gene ontology and pathway analysis showed that the DEGs were most enriched in the Piwi-interactiing RNA (piRNA) metabolic process, germ plasm, chromatoid body, P granule, neuroactive ligand-receptor interaction, microbial metabolism in diverse environments, and methane metabolism. A total of 111 DEGs, five gene ontology (GO) terms, and three pathways were significantly different between the left and right gonads among all the development stages. We also present the gene number and the percentage within eight development-dependent expression patterns of DEGs in the left and right gonads of female chicken. View Full-Text
Keywords: left-right asymmetry; gonads; female chicken; RNA-seq left-right asymmetry; gonads; female chicken; RNA-seq
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Wan, Z.; Lu, Y.; Rui, L.; Yu, X.; Yang, F.; Tu, C.; Li, Z. Gene Expression Profiling Reveals Potential Players of Left-Right Asymmetry in Female Chicken Gonads. Int. J. Mol. Sci. 2017, 18, 1299.

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