Next Article in Journal
Acute Effect of Metformin on Postprandial Hypertriglyceridemia through Delayed Gastric Emptying
Next Article in Special Issue
Antarctic Krill Oil Diet Protects against Lipopolysaccharide-Induced Oxidative Stress, Neuroinflammation and Cognitive Impairment
Previous Article in Journal
Targeting Autophagy in Cancer: Update on Clinical Trials and Novel Inhibitors
Article Menu
Issue 6 (June) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2017, 18(6), 1280; doi:10.3390/ijms18061280

GPE Promotes the Proliferation and Migration of Mouse Embryonic Neural Stem Cells and Their Progeny In Vitro

1
Department of Physiology, University of Santiago de Compostela, Santiago de Compostela 15710, Spain
2
Research and Development, Medical Center Foltra, Teo 15886, Spain
3
Scientific Direction, Medical Center Foltra, Teo 15886, Spain
*
Author to whom correspondence should be addressed.
Received: 6 May 2017 / Revised: 9 June 2017 / Accepted: 13 June 2017 / Published: 16 June 2017
(This article belongs to the Special Issue Neurological Injuries’ Monitoring, Tracking and Treatment 2017)
View Full-Text   |   Download PDF [4802 KB, uploaded 16 June 2017]   |  

Abstract

This study was designed to investigate a possible role of the N-terminal tripeptide of insulin-like growth factor-1 (IGF-I), Gly-Pro-Glu (GPE), physiologically generated in neurons following IGF-I-specific cleavage, in promoting neural regeneration after an injury. Primary cultures of mouse neural stem cells (NSCs), obtained from 13.5 Days post-conception (dpc) mouse embryos, were challenged with either GPE, growth hormone (GH), or GPE + GH and the effects on cell proliferation, migration, and survival were evaluated both under basal conditions and in response to a wound healing assay. The cellular pathways activated by GPE were also investigated by using specific chemical inhibitors. The results of the study indicate that GPE treatment promotes the proliferation and the migration of neural stem cells in vitro through a mechanism that involves the activation of extracellular signal-regulated kinase (ERK) and phosphoinositide 3-kinase PI3K-Akt pathways. Intriguingly, both GPE effects and the signaling pathways activated were similar to those observed after GH treatment. Based upon the results obtained from this study, GPE, as well as GH, may be useful in promoting neural protection and/or regeneration after an injury. View Full-Text
Keywords: GPE; GH; neural regeneration; ERK; PI3K/Akt; N-methyl-d-aspartate (NMDA); neural stem cells; brain injury GPE; GH; neural regeneration; ERK; PI3K/Akt; N-methyl-d-aspartate (NMDA); neural stem cells; brain injury
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Almengló, C.; Devesa, P.; Devesa, J.; Arce, V.M. GPE Promotes the Proliferation and Migration of Mouse Embryonic Neural Stem Cells and Their Progeny In Vitro. Int. J. Mol. Sci. 2017, 18, 1280.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top