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Int. J. Mol. Sci. 2017, 18(6), 1118; doi:10.3390/ijms18061118

Essential Role of Growth Hormone and IGF-1 in Therapeutic Effect of Ghrelin in the Course of Acetic Acid-Induced Colitis

1
Department of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 31-531 Cracow, Poland
2
Department of Pediatrics, Gastroenterology and Nutrition, University Children’s Hospital, Faculty of Medicine, Jagiellonian University Medical College, 30-663 Cracow, Poland
3
Department of Diagnostics, Chair of Clinical Biochemistry, Faculty of Medicine Jagiellonian University Medical College, 31-501 Cracow, Poland
4
Department of Medical Physiology Faculty of Health Sciences, Jagiellonian University Medical College, 31-126 Cracow, Poland
5
Department of Theory of Sport and Kinesiology, Faculty of Physical Education, University of Physical Education, 31-571 Cracow, Poland
6
Department of Pathophysiology, Faculty of Medicine, Jagiellonian University Medical College, 31-121 Cracow, Poland
7
Department of Pharmacology, Faculty of Medicine, Jagiellonian University Medical College, 31-531 Cracow, Poland
8
Department of Prosthetic Dentistry, Faculty of Medicine, Jagiellonian University Medical College, 31-155 Cracow, Poland
*
Author to whom correspondence should be addressed.
Academic Editors: Steve Harvey and Carlos G. Martinez-Moreno
Received: 5 April 2017 / Revised: 16 May 2017 / Accepted: 18 May 2017 / Published: 24 May 2017
(This article belongs to the Special Issue Growth Hormone: Therapeutic Possibilities)
View Full-Text   |   Download PDF [984 KB, uploaded 24 May 2017]   |  

Abstract

Previous studies have shown that ghrelin exhibits a protective and therapeutic effect in the gut. The aim of the present study was to examine whether administration of ghrelin affects the course of acetic acid-induced colitis and to determine what is the role of growth hormone (GH) and insulin-like growth factor-1 (IGF-1) in this effect. In sham-operated or hypophysectomized male Wistar rats, colitis was induced by enema with 1 mL of 3% solution of acetic acid. Saline or ghrelin (given at the dose of 8 nmol/kg/dose) was administered intraperitoneally twice a day. Seven days after colitis induction, rats were anesthetized and the severity of the colitis was assessed. Treatment with ghrelin reduced the area of colonic mucosa damage in pituitary-intact rat. This effect was associated with increase in serum levels of GH and IGF-1. Moreover, administration of ghrelin improved blood flow in colonic mucosa and mucosal cell proliferation, as well as reduced mucosal concentration of proinflammatory interleukin-1β (IL-1β) and activity of myeloperoxidase. Hypophysectomy reduced serum levels of GH and IGF-1 and increased the area of colonic damage in rats with colitis. These effects were associated with additional reduction in mucosal blood follow and DNA synthesis when compared to pituitary-intact rats. Mucosal concentration of IL-1β and mucosal activity of myeloperoxidase were maximally increased. Moreover, in hypophysectomized rats, administration of ghrelin failed to affect serum levels of GH or IGF-1, as well as the healing rate of colitis, mucosal cell proliferation, and mucosal concentration of IL-1β, or activity of myeloperoxidase. We conclude that administration of ghrelin accelerates the healing of the acetic acid-induced colitis. Therapeutic effect of ghrelin in experimental colitis is mainly mediated by the release of endogenous growth hormone and IGF-1. View Full-Text
Keywords: growth hormone; ghrelin; insulin-like growth factor-1; colitis; the pituitary gland growth hormone; ghrelin; insulin-like growth factor-1; colitis; the pituitary gland
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Ceranowicz, P.; Warzecha, Z.; Cieszkowski, J.; Ceranowicz, D.; Kuśnierz-Cabala, B.; Bonior, J.; Jaworek, J.; Ambroży, T.; Gil, K.; Olszanecki, R.; Pihut, M.; Dembiński, A. Essential Role of Growth Hormone and IGF-1 in Therapeutic Effect of Ghrelin in the Course of Acetic Acid-Induced Colitis. Int. J. Mol. Sci. 2017, 18, 1118.

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