Dual-Component Gelatinous Peptide/Reactive Oligomer Formulations as Conduit Material and Luminal Filler for Peripheral Nerve Regeneration
AbstractToward the next generation of nerve guidance conduits (NGCs), novel biomaterials and functionalization concepts are required to address clinical demands in peripheral nerve regeneration (PNR). As a biological polymer with bioactive motifs, gelatinous peptides are promising building blocks. In combination with an anhydride-containing oligomer, a dual-component hydrogel system (cGEL) was established. First, hollow cGEL tubes were fabricated by a continuous dosing and templating process. Conduits were characterized concerning their mechanical strength, in vitro and in vivo degradation and biocompatibility. Second, cGEL was reformulated as injectable shear thinning filler for established NGCs, here tyrosine-derived polycarbonate-based braided conduits. Thereby, the formulation contained the small molecule LM11A-31. The biofunctionalized cGEL filler was assessed regarding building block integration, mechanical properties, in vitro cytotoxicity, and growth permissive effects on human adipose tissue-derived stem cells. A positive in vitro evaluation motivated further application of the filler material in a sciatic nerve defect. Compared to the empty conduit and pristine cGEL, the functionalization performed superior, though the autologous nerve graft remains the gold standard. In conclusion, LM11A-31 functionalized cGEL filler with extracellular matrix (ECM)-like characteristics and specific biochemical cues holds great potential to support PNR. View Full-Text
- Supplementary File 1:
PDF-Document (PDF, 370 KB)
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Kohn-Polster, C.; Bhatnagar, D.; Woloszyn, D.J.; Richtmyer, M.; Starke, A.; Springwald, A.H.; Franz, S.; Schulz-Siegmund, M.; Kaplan, H.M.; Kohn, J.; Hacker, M.C. Dual-Component Gelatinous Peptide/Reactive Oligomer Formulations as Conduit Material and Luminal Filler for Peripheral Nerve Regeneration. Int. J. Mol. Sci. 2017, 18, 1104.
Kohn-Polster C, Bhatnagar D, Woloszyn DJ, Richtmyer M, Starke A, Springwald AH, Franz S, Schulz-Siegmund M, Kaplan HM, Kohn J, Hacker MC. Dual-Component Gelatinous Peptide/Reactive Oligomer Formulations as Conduit Material and Luminal Filler for Peripheral Nerve Regeneration. International Journal of Molecular Sciences. 2017; 18(5):1104.Chicago/Turabian Style
Kohn-Polster, Caroline; Bhatnagar, Divya; Woloszyn, Derek J.; Richtmyer, Matthew; Starke, Annett; Springwald, Alexandra H.; Franz, Sandra; Schulz-Siegmund, Michaela; Kaplan, Hilton M.; Kohn, Joachim; Hacker, Michael C. 2017. "Dual-Component Gelatinous Peptide/Reactive Oligomer Formulations as Conduit Material and Luminal Filler for Peripheral Nerve Regeneration." Int. J. Mol. Sci. 18, no. 5: 1104.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.