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Int. J. Mol. Sci. 2017, 18(5), 1002; doi:10.3390/ijms18051002

An Additive Effect of Promoting Thermogenic Gene Expression in Mice Adipose-Derived Stromal Vascular Cells by Combination of Rosiglitazone and CL316,243

1
Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, China
2
College of Animal Science and Technology, Gansu Agriculture University, Lanzhou 730070, Gansu, China
3
College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Giovanni Tarantino
Received: 29 March 2017 / Revised: 2 May 2017 / Accepted: 3 May 2017 / Published: 8 May 2017
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

It is well-documented that CL316,243 (a β3 agonist) or rosiglitazone (a PPARγ agonist) can induce white adipocyte populations to brown-like adipocytes, thus increasing energy consumption and combating obesity. However, whether there is a combined effect remains unknown. In the present study, stromal vascular cells of inguinal white adipose tissue (iWAT-SVCs for short) from mice were cultured and induced into browning by CL316,243, rosiglitazone, or both. Results showed that a combination of CL316,243 and rosiglitazone significantly upregulated the expression of the core thermogenic gene Ucp1 as well as genes related with mitochondrial function (Cidea, Cox5b, Cox7a1, Cox8b, and Cycs), compared with the treatment of CL316,243 or rosiglitazone alone. Moreover, co-treatment with rosiglitazone could reverse the downregulation of Adiponectin resulting from CL316,243 stimuli alone. Taken together, a combination of rosiglitazone and CL316,243 can produce an additive effect of promoting thermogenic gene expression and an improvement of insulin sensitivity in mouse iWAT-SVCs. View Full-Text
Keywords: adipocyte browning; rosiglitazone; CL316,243; beige; brite; thermogenic gene; additive effect adipocyte browning; rosiglitazone; CL316,243; beige; brite; thermogenic gene; additive effect
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Li, Y.-L.; Li, X.; Jiang, T.-T.; Fan, J.-M.; Zheng, X.-L.; Shi, X.-E.; Yu, T.-Y.; Chu, G.-Y.; Yang, G.-S. An Additive Effect of Promoting Thermogenic Gene Expression in Mice Adipose-Derived Stromal Vascular Cells by Combination of Rosiglitazone and CL316,243. Int. J. Mol. Sci. 2017, 18, 1002.

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