Identification of Novel A2/C2 Inter-Genotype Recombinants of Hepatitis B Virus from a Korean Chronic Patient Co-Infected with Both Genotype A2 and C2
Abstract
:1. Introduction
2. Results
2.1. Identification of Inter-Genotypic Recombinants of Genotype A2/C2 from a Korean Patient, H62, by Phylogenetic Analysis of 1032-bp Polymerase RT Sequences
2.2. Identification of Recombinant Breakpoints in Four Recombinant Clones by BootScan Analysis
2.3. The Simultaneous Modification of HBsAg by Recombination in the Polymerase RT Region
3. Discussion
4. Materials and Methods
4.1. Patient
4.2. DNA Extraction and Amplification of the Polymerase RT Region
4.3. TA Cloning and Sequencing Analysis of the Amplicon
4.4. HBV Genotyping
4.5. BootScan Analysis
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Lee, S.-Y.; Lee, S.-H.; Kim, J.-E.; Kim, H.; Kim, K.; Kook, Y.-H.; Kim, B.-J. Identification of Novel A2/C2 Inter-Genotype Recombinants of Hepatitis B Virus from a Korean Chronic Patient Co-Infected with Both Genotype A2 and C2. Int. J. Mol. Sci. 2017, 18, 737. https://doi.org/10.3390/ijms18040737
Lee S-Y, Lee S-H, Kim J-E, Kim H, Kim K, Kook Y-H, Kim B-J. Identification of Novel A2/C2 Inter-Genotype Recombinants of Hepatitis B Virus from a Korean Chronic Patient Co-Infected with Both Genotype A2 and C2. International Journal of Molecular Sciences. 2017; 18(4):737. https://doi.org/10.3390/ijms18040737
Chicago/Turabian StyleLee, So-Young, Seung-Hee Lee, Ji-Eun Kim, Hong Kim, Kijeong Kim, Yoon-Hoh Kook, and Bum-Joon Kim. 2017. "Identification of Novel A2/C2 Inter-Genotype Recombinants of Hepatitis B Virus from a Korean Chronic Patient Co-Infected with Both Genotype A2 and C2" International Journal of Molecular Sciences 18, no. 4: 737. https://doi.org/10.3390/ijms18040737