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Int. J. Mol. Sci. 2017, 18(4), 737; doi:10.3390/ijms18040737

Identification of Novel A2/C2 Inter-Genotype Recombinants of Hepatitis B Virus from a Korean Chronic Patient Co-Infected with Both Genotype A2 and C2

1
Department of Biomedical Sciences, Microbiology and Immunology, Liver Research Institute and Cancer Research Institute, College of Medicine, Seoul National University, 28 Yongon-dong, Chongno-gu, Seoul 110-799, Korea
2
Department of Microbiology, School of Medicine, Chung-Ang University, Seoul 156-756, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Susanna K. P. Lau
Received: 17 January 2017 / Revised: 21 March 2017 / Accepted: 27 March 2017 / Published: 30 March 2017
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Abstract

Nearly all cases of Hepatitis B virus (HBV) infections in South Korea have the C2 genotype. Here, we have identified a chronically infected patient who was co-infected with HBV of both the A2 and C2 genotypes by screening 135 Korean chronically infected patients using direct sequencing protocols targeting the 1032-bp polymerase reverse transcriptase (RT) region. Further polymerase chain reaction (PCR)-cloning analysis (22 clones) of the RT showed that this patient had genotype C2 (12 clones), genotype A2 (six clones) and A2/C2 inter-genotype HBV recombinants (four clones). BootScan analysis showed that three of the four recombinants have different types of recombination breakpoints in both the RT and overlapping hepatitis B surface antigen (HBsAg) region. Given the significance of HBsAg as a diagnostic or vaccination target against HBV infection, clinical implications of these identified recombinants should be studied in the future. To our knowledge, this is the first report on A2/C2 inter-genotype HBV recombinants. View Full-Text
Keywords: Hepatitis B virus; polymerase RT region; small surface protein; subgenotype A2; subgenotype C2; inter-genotypical recombination Hepatitis B virus; polymerase RT region; small surface protein; subgenotype A2; subgenotype C2; inter-genotypical recombination
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Lee, S.-Y.; Lee, S.-H.; Kim, J.-E.; Kim, H.; Kim, K.; Kook, Y.-H.; Kim, B.-J. Identification of Novel A2/C2 Inter-Genotype Recombinants of Hepatitis B Virus from a Korean Chronic Patient Co-Infected with Both Genotype A2 and C2. Int. J. Mol. Sci. 2017, 18, 737.

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