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Int. J. Mol. Sci. 2017, 18(4), 727; doi:10.3390/ijms18040727

MicroRNA-17-92 Regulates the Transcription Factor E2F3b during Myogenesis In Vitro and In Vivo

1
Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences, Shenzhen University, Shenzhen 518060, China
2
Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Shenzhen University Health Sciences Center, Shenzhen 518000, China
3
School of Life Sciences, Peking University, Beijing 100871, China
4
Biomedical Engineering, College of Health and Environmental Engineering, Shenzhen Technology University, Shenzhen 51000, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editors: Julian Borejdo and William Chi-shing Cho
Received: 31 December 2016 / Revised: 11 March 2017 / Accepted: 24 March 2017 / Published: 31 March 2017
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
View Full-Text   |   Download PDF [2337 KB, uploaded 31 March 2017]   |  

Abstract

Myogenic differentiation, which occurs during muscle development, is a highly ordered process that can be regulated by E2F transcription factors. Available data show that E2F3b, but not E2F3a, is upregulated and required for myogenic differentiation. However, the regulation of E2F3b expression in myogenic differentiation is not well understood. To investigate whether E2Fb expression is controlled by miRNAs, we used bioinformatics to combine the database of microRNAs downregulated during myogenesis and those predicted to target E2F3. This identified miR-17 and miR-20a as miRNAs potentially involved in E2F3 regulation. We found that miR-17-92 controls the expression of E2F3b in C2C12 cells during myogenic differentiation. Moreover, we confirmed that miR-20a regulates the expression of E2F3b proteins in vivo using a muscle regeneration model. View Full-Text
Keywords: miR-20a; E2F3b; muscle miR-20a; E2F3b; muscle
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Tang, Z.; Liu, N.; Luo, L.; Kang, K.; Li, L.; Ni, R.; Qiu, H.; Gou, D. MicroRNA-17-92 Regulates the Transcription Factor E2F3b during Myogenesis In Vitro and In Vivo. Int. J. Mol. Sci. 2017, 18, 727.

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