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Int. J. Mol. Sci. 2017, 18(3), 610; doi:10.3390/ijms18030610

The Application of Curve Fitting on the Voltammograms of Various Isoforms of Metallothioneins–Metal Complexes

1
Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic
2
Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic
3
Department of Physiology, Institute of Nutrition and Food Technology, University of Granada, Avenida Del Conocimiento S/N Biomedical Research Centre, Health Campus, 18001 Granada, Spain
4
Department of Paediatric Haematology and Oncology, 2nd Faculty of Medicine, Charles University and University Hospital Motol, V Uvalu 84, CZ-150 06 Prague 5, Czech Republic
*
Author to whom correspondence should be addressed.
Academic Editor: Eva Freisinger
Received: 11 February 2017 / Revised: 3 March 2017 / Accepted: 7 March 2017 / Published: 11 March 2017
(This article belongs to the Special Issue Metallothioneins in Bioinorganic Chemistry: Recent Developments)
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Abstract

The translation of metallothioneins (MTs) is one of the defense strategies by which organisms protect themselves from metal-induced toxicity. MTs belong to a family of proteins comprising MT-1, MT-2, MT-3, and MT-4 classes, with multiple isoforms within each class. The main aim of this study was to determine the behavior of MT in dependence on various externally modelled environments, using electrochemistry. In our study, the mass distribution of MTs was characterized using MALDI-TOF. After that, adsorptive transfer stripping technique with differential pulse voltammetry was selected for optimization of electrochemical detection of MTs with regard to accumulation time and pH effects. Our results show that utilization of 0.5 M NaCl, pH 6.4, as the supporting electrolyte provides a highly complicated fingerprint, showing a number of non-resolved voltammograms. Hence, we further resolved the voltammograms exhibiting the broad and overlapping signals using curve fitting. The separated signals were assigned to the electrochemical responses of several MT complexes with zinc(II), cadmium(II), and copper(II), respectively. Our results show that electrochemistry could serve as a great tool for metalloproteomic applications to determine the ratio of metal ion bonds within the target protein structure, however, it provides highly complicated signals, which require further resolution using a proper statistical method, such as curve fitting. View Full-Text
Keywords: electrochemistry; mass spectrometry MALDI-TOF; metallothionein; metallomics; signal resolving electrochemistry; mass spectrometry MALDI-TOF; metallothionein; metallomics; signal resolving
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Merlos Rodrigo, M.A.; Molina-López, J.; Jimenez Jimenez, A.M.; Planells Del Pozo, E.; Adam, P.; Eckschlager, T.; Zitka, O.; Richtera, L.; Adam, V. The Application of Curve Fitting on the Voltammograms of Various Isoforms of Metallothioneins–Metal Complexes. Int. J. Mol. Sci. 2017, 18, 610.

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