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Int. J. Mol. Sci. 2017, 18(3), 543; doi:10.3390/ijms18030543

Simplifying the Preparation of Pollen Grains for MALDI-TOF MS Classification

1
Department of Chemistry, Humboldt-University to Berlin, Brook-Taylor-Str. 2, 12489 Berlin, Germany
2
Federal Institute for Materials Research and Testing (BAM), Richard-Willstätter-Str. 11, 12489 Berlin, Germany
3
Present Address: Institute of Medical Informatics and Statistics, Kiel University, Brunswiker Str. 10, 24105 Kiel, Germany
*
Author to whom correspondence should be addressed.
Academic Editors: David Arráez-Román and Ana Maria Gómez-Caravaca
Received: 31 January 2017 / Revised: 24 February 2017 / Accepted: 27 February 2017 / Published: 3 March 2017
(This article belongs to the Special Issue Analytical Techniques in Plant and Food Analysis)
View Full-Text   |   Download PDF [2408 KB, uploaded 3 March 2017]   |  

Abstract

Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) is a well-implemented analytical technique for the investigation of complex biological samples. In MS, the sample preparation strategy is decisive for the success of the measurements. Here, sample preparation processes and target materials for the investigation of different pollen grains are compared. A reduced and optimized sample preparation process prior to MALDI-TOF measurement is presented using conductive carbon tape as target. The application of conductive tape yields in enhanced absolute signal intensities and mass spectral pattern information, which leads to a clear separation in subsequent pattern analysis. The results will be used to improve the taxonomic differentiation and identification, and might be useful for the development of a simple routine method to identify pollen based on mass spectrometry. View Full-Text
Keywords: MALDI-TOF MS; conductive carbon tape; pollen; sample pretreatment; principal component analysis MALDI-TOF MS; conductive carbon tape; pollen; sample pretreatment; principal component analysis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Lauer, F.; Seifert, S.; Kneipp, J.; Weidner, S.M. Simplifying the Preparation of Pollen Grains for MALDI-TOF MS Classification. Int. J. Mol. Sci. 2017, 18, 543.

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