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Int. J. Mol. Sci. 2017, 18(3), 480; doi:10.3390/ijms18030480

Intracellular Accumulation of Methylglyoxal by Glyoxalase 1 Knock Down Alters Collagen Homoeostasis in L6 Myoblasts

1
Herz- and Diabeteszentrum NRW, Diabeteszentrum, Ruhr Universität Bochum, 32545 Bad Oeynhausen, Germany
2
Warwick Medical School, Clinical Sciences Research Laboratories, University of Warwick, University Hospital, Coventry CV2 2DX, UK
*
Author to whom correspondence should be addressed.
Academic Editor: Casper G. Schalkwijk
Received: 2 December 2016 / Revised: 6 February 2017 / Accepted: 17 February 2017 / Published: 23 February 2017
(This article belongs to the Special Issue Glyoxalase System)
View Full-Text   |   Download PDF [1743 KB, uploaded 23 February 2017]   |  

Abstract

Hyperglycemia results in accumulation of the reactive dicarbonyl methylglyoxal (MG). Methylglyoxal is detoxified by the glyoxalase system (glyoxalase 1 and 2). The influence of glyoxalase 1 knockdown on expression of collagens 1, 3, 4, and 5 in L6 myoblasts under hyperglycemic conditions was investigated. Increased biosynthesis of collagens 1, 3, 4, and 5 was detected at mRNA-level following knockdown of glyoxalase 1 (GLO1). At the protein level a significant elevation of the concentration of collagen 1 and 4 was shown, whereas no increase of collagen 5 and a non-significant increase in collagen 3 were detectable. These results could partially explain MG-induced changes in the extracellular matrix (ECM) which account for increased fibrosis and impaired function in myocytes. The mechanisms by which reactive glucose metabolites influence ECM composition deserve further investigation. View Full-Text
Keywords: hyperglycemia; dicarbonyl proteome; methylglyoxal; collagen; glyoxalase 1 hyperglycemia; dicarbonyl proteome; methylglyoxal; collagen; glyoxalase 1
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MDPI and ACS Style

Stratmann, B.; Goldstein, B.; Thornalley, P.J.; Rabbani, N.; Tschoepe, D. Intracellular Accumulation of Methylglyoxal by Glyoxalase 1 Knock Down Alters Collagen Homoeostasis in L6 Myoblasts. Int. J. Mol. Sci. 2017, 18, 480.

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