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Int. J. Mol. Sci. 2017, 18(2), 446; doi:10.3390/ijms18020446

Time, Concentration, and pH-Dependent Transport and Uptake of Anthocyanins in a Human Gastric Epithelial (NCI-N87) Cell Line

1
Department of Food Science and Technology, The Ohio State University, Columbus, OH 43210, USA
2
Department of Human Nutrition, The Ohio State University, Columbus, OH 43210, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Nuno Mateus and Iva Fernandes
Received: 30 December 2016 / Revised: 13 February 2017 / Accepted: 16 February 2017 / Published: 18 February 2017
(This article belongs to the Special Issue Anthocyanins)
View Full-Text   |   Download PDF [1225 KB, uploaded 18 February 2017]   |  

Abstract

Anthocyanins are the largest class of water soluble plant pigments and a common part of the human diet. They may have many potential health benefits, including antioxidant, anti-inflammatory, anti-cancer, and cardioprotective activities. However, anthocyanin metabolism is not well understood. Studies suggest that anthocyanins absorption may occur in the stomach, in which the acidic pH favors anthocyanin stability. A gastric epithelial cell line (NCI-N87) has been used to study the behavior of anthocyanins at a pH range of 3.0–7.4. This work examines the effects of time (0–3 h), concentration (50–1500 µM), and pH (3.0, 5.0, 7.4) on the transport and uptake of anthocyanins using NCI-N87 cells. Anthocyanins were transported from the apical to basolateral side of NCI-N87 cells in time and dose dependent manners. Over the treatment time of 3 h the rate of transport increased, especially with higher anthocyanin concentrations. The non-linear rate of transport may suggest an active mechanism for the transport of anthocyanins across the NCI-N87 monolayer. At apical pH 3.0, higher anthocyanin transport was observed compared to pH 5.0 and 7.4. Reduced transport of anthocyanins was found to occur at apical pH 5.0. View Full-Text
Keywords: anthocyanins; gastric; stomach; uptake; NCI-N87 cell line; chokeberry anthocyanins; gastric; stomach; uptake; NCI-N87 cell line; chokeberry
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Atnip, A.A.; Sigurdson, G.T.; Bomser, J.; Giusti, M.M. Time, Concentration, and pH-Dependent Transport and Uptake of Anthocyanins in a Human Gastric Epithelial (NCI-N87) Cell Line. Int. J. Mol. Sci. 2017, 18, 446.

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