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Int. J. Mol. Sci. 2017, 18(2), 345; doi:10.3390/ijms18020345

Micro-Computed Tomography Detection of Gold Nanoparticle-Labelled Mesenchymal Stem Cells in the Rat Subretinal Layer

1
Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
2
Genetics and Regenerative Medicine Research Center, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
3
Department of Ophthalmology, Hospital Sultanah Aminah, 80100 Johor Bahru, Johor, Malaysia
4
Department of Ophthalmology, Faculty of Medicine, UKM Medical Center, 56000 Cheras, Kuala Lumpur, Malaysia
5
Centre for Eye Research Australia, Royal Victorian Eye & Ear Hospital, Melbourne 3002, Australia
6
Department of Surgery (Ophthalmology), The University of Melbourne, Melbourne 3002, Australia
7
Tissue Engineering Centre, Universiti Kebangsaan Malaysia Medical Center, 56000 Cheras, Kuala Lumpur, Malaysia
8
Department of Physiology, Universiti Kebangsaan Malaysia Medical Center, 56000 Cheras, Kuala Lumpur, Malaysia
9
Department of Medical Microbiology and Parasitology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
10
Department of Chemical and Materials Engineering, National Central University, Jhong-li, Taoyuan 32001, Taiwan
11
Department of Reproduction, National Research Institute for Child Health and Development, Tokyo 157-8535, Japan
12
Department of Botany and Microbiology, King Saud University, Riyadh 11451, Saudi Arabia
*
Authors to whom correspondence should be addressed.
Academic Editors: Adriano Piatelli and Barbara Zavan
Received: 11 November 2016 / Revised: 9 December 2016 / Accepted: 21 December 2016 / Published: 8 February 2017
View Full-Text   |   Download PDF [19830 KB, uploaded 8 February 2017]   |  

Abstract

Mesenchymal stem cells are widely used in many pre-clinical and clinical settings. Despite advances in molecular technology; the migration and homing activities of these cells in in vivo systems are not well understood. Labelling mesenchymal stem cells with gold nanoparticles has no cytotoxic effect and may offer suitable indications for stem cell tracking. Here, we report a simple protocol to label mesenchymal stem cells using 80 nm gold nanoparticles. Once the cells and particles were incubated together for 24 h, the labelled products were injected into the rat subretinal layer. Micro-computed tomography was then conducted on the 15th and 30th day post-injection to track the movement of these cells, as visualized by an area of hyperdensity from the coronal section images of the rat head. In addition, we confirmed the cellular uptake of the gold nanoparticles by the mesenchymal stem cells using transmission electron microscopy. As opposed to other methods, the current protocol provides a simple, less labour-intensive and more efficient labelling mechanism for real-time cell tracking. Finally, we discuss the potential manipulations of gold nanoparticles in stem cells for cell replacement and cancer therapy in ocular disorders or diseases. View Full-Text
Keywords: mesenchymal stem cells; gold nanoparticles; stem cell tracking; rat subretinal layer; transmission electron microscopy; micro-computed tomography mesenchymal stem cells; gold nanoparticles; stem cell tracking; rat subretinal layer; transmission electron microscopy; micro-computed tomography
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MDPI and ACS Style

Mok, P.L.; Leow, S.N.; Koh, A.E.-H.; Mohd Nizam, H.H.; Ding, S.L.S.; Luu, C.; Ruhaslizan, R.; Wong, H.S.; Halim, W.H.W.A.; Ng, M.H.; Idrus, R.B.H.; Chowdhury, S.R.; Bastion, C.M.-L.; Subbiah, S.K.; Higuchi, A.; Alarfaj, A.A.; Then, K.Y. Micro-Computed Tomography Detection of Gold Nanoparticle-Labelled Mesenchymal Stem Cells in the Rat Subretinal Layer. Int. J. Mol. Sci. 2017, 18, 345.

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