Plumbagin Suppresses α-MSH-Induced Melanogenesis in B16F10 Mouse Melanoma Cells by Inhibiting Tyrosinase Activity
Abstract
:1. Introduction
2. Results
2.1. Chemical Structure and Cytotoxic Effects of Plumbagin in B16F10 Mouse Melanoma Cells
2.2. Plumbagin Suppresses α-MSH-Induced Melanin Synthesis in B16F10 Mouse Melanoma Cells
2.3. Plumbagin Does Not Regulate α-MSH-Induced Melanogenesis Gene Expression and Signal Transduction Cascades
2.4. Plumbagin Inhibits Tyrosinase Activity
2.5. Plumbagin Is Not Toxic in Normal Keratinocytes and Lens Epithelial Cells
3. Discussion
4. Materials and Methods
4.1. Reagents and Antibodies
4.2. Cell Culture and Cell Viability Assay
4.3. Immunoblotting
4.4. Measurement of Intracellular and Extracellular Melanin Content
4.5. RNA Isolation and Quantitative RT-PCR
4.6. Cellular Tyrosinase Activity Assay
4.7. Cell-Free Tyrosinase Activity Assay
4.8. DPPH Radical Scavenging Activity Assay
4.9. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
α-MSH | alpha-melanocyte stimulating hormone |
TYR | tyrosinase |
l-DOPA | l-3,4-dihydroxyphenylalanine |
MITF | microphthalmia-associated transcription factor |
TYRP1 | tyrosinase-related protein 1 |
CREB | cAMP response element binding protein |
PKA | protein kinase A |
ERK1/2 | extracellular signal-regulated kinase 1/2 |
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Oh, T.-I.; Yun, J.-M.; Park, E.-J.; Kim, Y.-S.; Lee, Y.-M.; Lim, J.-H. Plumbagin Suppresses α-MSH-Induced Melanogenesis in B16F10 Mouse Melanoma Cells by Inhibiting Tyrosinase Activity. Int. J. Mol. Sci. 2017, 18, 320. https://doi.org/10.3390/ijms18020320
Oh T-I, Yun J-M, Park E-J, Kim Y-S, Lee Y-M, Lim J-H. Plumbagin Suppresses α-MSH-Induced Melanogenesis in B16F10 Mouse Melanoma Cells by Inhibiting Tyrosinase Activity. International Journal of Molecular Sciences. 2017; 18(2):320. https://doi.org/10.3390/ijms18020320
Chicago/Turabian StyleOh, Taek-In, Jeong-Mi Yun, Eun-Ji Park, Young-Seon Kim, Yoon-Mi Lee, and Ji-Hong Lim. 2017. "Plumbagin Suppresses α-MSH-Induced Melanogenesis in B16F10 Mouse Melanoma Cells by Inhibiting Tyrosinase Activity" International Journal of Molecular Sciences 18, no. 2: 320. https://doi.org/10.3390/ijms18020320