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Int. J. Mol. Sci. 2017, 18(2), 282; doi:10.3390/ijms18020282

α6 Integrin (α6high)/Transferrin Receptor (CD71)low Keratinocyte Stem Cells Are More Potent for Generating Reconstructed Skin Epidermis Than Rapid Adherent Cells

1
Gattefossé, 36 chemin de Genas, F-69800 Saint-Priest, France
2
Commissariat à l'énergie atomique et aux énergies alternatives (CEA)/Institut Nanosciences et cryogénie (INAC)/SYstèmes Moléculaires et nanoMatériaux pour l’Energie et la Santé (SyMMES)/Lésions des acides nucléiques (LAN), 17 avenue des martyrs, F-38054 Grenoble CEDEX, France
3
Hospices Civils de LYON (HCL)/Banque de Tissus et Cellules/Laboratoire des Substituts Cutanés, 5 place d’Arsonval, F-69000 Lyon, France
4
Department of Biological Sciences, University Grenoble Alpes, F-38000 Grenoble, France
*
Author to whom correspondence should be addressed.
Academic Editor: Ritva Tikkanen
Received: 15 December 2016 / Revised: 8 January 2017 / Accepted: 16 January 2017 / Published: 27 January 2017
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
View Full-Text   |   Download PDF [2676 KB, uploaded 3 February 2017]   |  

Abstract

The epidermis basal layer is composed of two keratinocyte populations: Keratinocyte Stem cells (KSC) and Transitory Amplifying (TA) cells that arise from KSC division. Unfortunately, no specific marker exists to differ between KSC and TA cells. Here, we aimed at comparing two different methods that pretended to isolate these two populations: (i) the rapid adhesion method on coated substrate and (ii) the flow cytometry method, which is based on the difference in cell surface expressions of the α6 integrin and transferrin receptor (CD71). Then, we compared different parameters that are known to discriminate KSC and TA populations. Interestingly, we showed that both methods allow enrichment in stem cells. However, cell sorting by flow cytometry (α6high/CD71low) phenotype leads to a better enrichment of KSC since the colony forming efficiency is five times increased versus total cell suspension, whereas it is only 1.4 times for the adhesion method. Moreover, α6high/CD71low cells give rise to a thicker pluristratified epithelium with lower seeding density and display a low Ki67 positive cells number, showing that they have reached the balance between proliferation and differentiation. We clearly demonstrated that cells isolated by a rapid adherent method are not the same population as KSC isolated by flow cytometry following α6high/CD71low phenotype. View Full-Text
Keywords: skin; epidermis; basal keratinocytes; keratinocyte stem cells; adhesion; α6 integrin; CD71 skin; epidermis; basal keratinocytes; keratinocyte stem cells; adhesion; α6 integrin; CD71
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Metral, E.; Bechetoille, N.; Demarne, F.; Rachidi, W.; Damour, O. α6 Integrin (α6high)/Transferrin Receptor (CD71)low Keratinocyte Stem Cells Are More Potent for Generating Reconstructed Skin Epidermis Than Rapid Adherent Cells. Int. J. Mol. Sci. 2017, 18, 282.

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