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Int. J. Mol. Sci. 2017, 18(12), 2754; https://doi.org/10.3390/ijms18122754

Flavonoids of Kudzu Root Fermented by Eurtotium cristatum Protected Rat Pheochromocytoma Line 12 (PC12) Cells against H2O2-Induced Apoptosis

1
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
2
College of Food Science and Technology, Bohai University, Jinzhou 121013, China
3
Jiangsu Key Construction Laboratory of Food Resource Development and Quality Safe, Xuzhou Institute Technology, Xuzhou 221008, China
*
Author to whom correspondence should be addressed.
Received: 20 November 2017 / Revised: 15 December 2017 / Accepted: 15 December 2017 / Published: 19 December 2017
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Novel bioactive components have greatly attracted attention as they demonstrate health benefits. Reversed-phase high performance liquid chromatography (RP-HPLC) showed that isoflavonoid compounds of kudzu root (Pueraria lobata) fermented by Eurtotium cristatum and extracted using de-ionized water were higher active compared with non-fermented. A model of H2O2-inducd cell damage was built using rat pheochromocytoma line 12 (PC12) cell to observe the protective effect of non-fermented kudzu root (Pueraria lobata) (NFK) and fermented kudzu root (Pueraria lobata) (FK). Cell viability and apoptosis were analyzed through inverted microscopy and flow cytometry. The level of lactate dehydrogenase, catalase activity, superoxide dismutase, glutathione, and reactive oxygen species (ROS) were evaluated. Results showed that NFK and FK could significantly protect PC12 cell against damage caused by H2O2-induced oxidative stress. The intracellular antioxidant system was increased, protected the cell membrane inhibit H2O2-induced apoptosis by scavenging of ROS. Moreover, NFK and FK regulated the cell cycle to prevent cell apoptosis. Isoflavonoid from the kudzu root especially fermented kudzu root with E. cristatum are potentially therapeutic drugs against diseases induced by oxidative damage. View Full-Text
Keywords: kudzu root (Pueraria lobata); PC12 cell; H2O2-induced damage; reactive oxygen species; apoptosis kudzu root (Pueraria lobata); PC12 cell; H2O2-induced damage; reactive oxygen species; apoptosis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Zhang, B.; Li, W.; Dong, M. Flavonoids of Kudzu Root Fermented by Eurtotium cristatum Protected Rat Pheochromocytoma Line 12 (PC12) Cells against H2O2-Induced Apoptosis. Int. J. Mol. Sci. 2017, 18, 2754.

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