Specific Effects of Chronic Dietary Exposure to Chlorpyrifos on Brain Gene Expression—A Mouse Study
Abstractchlorpyrifos (CPF) is an organophosphate insecticide used to control pests on a variety of food and feed crops. In mammals, maternal exposure to CPF has been reported to induce cerebral cortex thinning, alteration of long-term brain cognitive function, and Parkinson-like symptoms, but the mechanisms of these processes are not fully understood. In this study, we aimed to gain a deeper understanding of the alterations induced in the brains of mice chronically exposed to CPF by dietary intake. For our purpose, we analysed F1 offspring (sacrificed at 3 and 8 months) of Mus musculus, treated in utero and postnatally with 3 different doses of CPF (0.1-1-10 mg/kg/day). Using RT2 Profiler PCR Arrays, we evaluated the alterations in the expression of 84 genes associated with neurodegenerative diseases. In the brains of exposed mice, we evidenced a clear dose–response relationship for AChE inhibition and alterations of gene expression. Some of the genes that were steadily down-regulated, such as Pink1, Park 2, Sv2b, Gabbr2, Sept5 and Atxn2, were directly related to Parkinson’s onset. Our experimental results shed light on the possibility that long-term CPF exposure may exert membrane signalling alterations which make brain cells more susceptible to develop neurodegenerative diseases. View Full-Text
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Pallotta, M.M.; Ronca, R.; Carotenuto, R.; Porreca, I.; Turano, M.; Ambrosino, C.; Capriglione, T. Specific Effects of Chronic Dietary Exposure to Chlorpyrifos on Brain Gene Expression—A Mouse Study. Int. J. Mol. Sci. 2017, 18, 2467.
Pallotta MM, Ronca R, Carotenuto R, Porreca I, Turano M, Ambrosino C, Capriglione T. Specific Effects of Chronic Dietary Exposure to Chlorpyrifos on Brain Gene Expression—A Mouse Study. International Journal of Molecular Sciences. 2017; 18(11):2467.Chicago/Turabian Style
Pallotta, Maria M.; Ronca, Raffaele; Carotenuto, Rosa; Porreca, Immacolata; Turano, Mimmo; Ambrosino, Concetta; Capriglione, Teresa. 2017. "Specific Effects of Chronic Dietary Exposure to Chlorpyrifos on Brain Gene Expression—A Mouse Study." Int. J. Mol. Sci. 18, no. 11: 2467.
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