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Int. J. Mol. Sci. 2017, 18(11), 2425; https://doi.org/10.3390/ijms18112425

TRPV3 Channel in Keratinocytes in Scars with Post-Burn Pruritus

1
Department of Dermatology, Kangnam Sacred Heart Hospital, Hallym University, 1 Singil-ro, Seoul 07441, Korea
2
Department of Microbiology, School of Medicine, Ewha Womans University, 911-1 Mok-Dong, Yang Cheon-Gu, Seoul 158-710, Korea
3
Department of Pharmacology, College of Medicine, Institute of Natural Medicine, Hallym University, Chunchon 200-702, Korea
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 4 October 2017 / Revised: 10 November 2017 / Accepted: 11 November 2017 / Published: 15 November 2017
(This article belongs to the Special Issue Recent Advances in Scar Biology)
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Abstract

Post-burn pruritus is a common and distressing sequela of burn scars. Empirical antipruritic treatments usually fail to have a satisfactory outcome because of their limited selectivity and possible side effects. Therefore, novel drug targets need to be identified. Here, we aimed to investigate the possible role of protease-activated receptor 2 (PAR2) and transient receptor potential vanniloid 3 (TRPV3), along with the relation of TRPV3 to thymic stromal lymphopoietin (TSLP). Specimens from normal (unscarred) or burn-scarred (with or without pruritus) tissue were obtained from burn patients for this study. In each sample, the keratinocytes were isolated and cultured, and the intracellular Ca2+ level at the time of stimulation of each factor was quantified and the interaction was screened. PAR2 function was reduced by antagonism of TRPV3. Inhibiting protein kinase A (PKA) and protein kinase C (PKC) reduced TRPV3 function. TSLP mRNA and protein, and TSLPR protein expressions, increased in scars with post-burn pruritus, compared to scars without it or to normal tissues. In addition, TRPV1 or TRPV3 activation induced increased TSLP expression. Conclusively, TRPV3 may contribute to pruritus in burn scars through TSLP, and can be considered a potential therapeutic target for post-burn pruritus. View Full-Text
Keywords: protease-activated receptor 2; transient receptor potential vanniloid 3; thymic stromal lymphopoietin; post-burn pruritus protease-activated receptor 2; transient receptor potential vanniloid 3; thymic stromal lymphopoietin; post-burn pruritus
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Park, C.W.; Kim, H.J.; Choi, Y.W.; Chung, B.Y.; Woo, S.-Y.; Song, D.-K.; Kim, H.O. TRPV3 Channel in Keratinocytes in Scars with Post-Burn Pruritus. Int. J. Mol. Sci. 2017, 18, 2425.

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