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Int. J. Mol. Sci. 2017, 18(11), 2372; https://doi.org/10.3390/ijms18112372

Glucosinolate-Derived Isothiocyanates Inhibit Arabidopsis Growth and the Potency Depends on Their Side Chain Structure

Cell, Molecular Biology and Genomics Group, Department of Biology, Norwegian University of Science and Technology, Høgskoleringen 5, NO-7491 Trondheim, Norway
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Received: 29 September 2017 / Revised: 1 November 2017 / Accepted: 3 November 2017 / Published: 8 November 2017
(This article belongs to the Section Molecular Plant Sciences)
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Abstract

Isothiocyanates (ITCs), the biologically important glucosinolate breakdown products, can present health-promoting effects, play an important role in plant defense and affect plant cellular mechanisms. Here, we evaluated the biological effects of ITCs on Arabidopsis thaliana by assessing growth parameters after long-term exposure to low concentrations of aliphatic and aromatic ITCs, ranging from 1 to 1000 µM. Treatment with the aliphatic allylisothiocyanate (allyl-ITC) led to a significant reduction of root length and fresh weight in a dose-dependent manner and affected the formation of lateral roots. To assess the importance of a hormonal crosstalk in the allyl-ITC-mediated growth reduction, the response of auxin and ethylene mutants was investigated, but our results did not allow us to confirm a role for these hormones. Aromatic ITCs generally led to a more severe growth inhibition than the aliphatic allyl-ITC. Interestingly, we observed a correlation between the length of their side chain and the effect these aromatic ITCs caused on Arabidopsis thaliana, with the greatest inhibitory effect seen for 2-phenylethyl-ITC. Root growth recovered when seedlings were removed from exposure to ITCs. View Full-Text
Keywords: Arabidopsis thaliana; glucosinolates; growth inhibition; isothiocyanates; physiological effects; secondary metabolites Arabidopsis thaliana; glucosinolates; growth inhibition; isothiocyanates; physiological effects; secondary metabolites
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Urbancsok, J.; Bones, A.M.; Kissen, R. Glucosinolate-Derived Isothiocyanates Inhibit Arabidopsis Growth and the Potency Depends on Their Side Chain Structure. Int. J. Mol. Sci. 2017, 18, 2372.

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