Hydrogel Film-Immobilized Lactobacillus brevis RK03 for γ-Aminobutyric Acid Production
Abstract
:1. Introduction
2. Results
2.1. Synthesis and Characterization of Polyacrylic Hydrogel Films
2.2. GABA Production by Immobilized Cells on Polyacrylic Hydrogel Films
2.3. GABA Production by Immobilized Cells on Polyacrylic Hydrogel Films Using Semi-Continuous Fermentation
2.4. Immobilization of L. brevis RK03 onto Polyacrylic Hydrogel Films
3. Discussion
4. Materials and Methods
4.1. Isolation and Identification of GABA-Producing LAB
4.2. Scanning Electron Microscopy of Lactobacillus brevis RK03
4.3. Preparation of Polyacrylic Hydrogel Films for Cell Immobilization
4.4. Tensile Strength and Water Content Measurement
4.5. Fourier Transform Infrared Spectroscopy (FTIR) Analysis
4.6. GABA and MSG Analysis
4.7. GABA and MSG Analysis in Semi-Continuous Conditions
4.8. Measurement of GABA Content with High-Performance Liquid Chromatography (HPLC)
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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No. | HEMA | PEGDA Mw: 200 | PEGDA Mw: 400 | PEGDA Mw: 700 | AA | Initiator | Tensile Strength (kg/cm2) | Water Content (%) |
---|---|---|---|---|---|---|---|---|
A | 95 | 3 | - | - | 1 | 1 | 9.31 | 31.41 |
B | 93 | 3 | - | - | 3 | 1 | 7.26 | 31.56 |
C | 91 | 3 | - | - | 5 | 1 | 6.61 | 32.45 |
D | 95 | - | 3 | - | 1 | 1 | 7.52 | 32.10 |
E | 93 | - | 3 | - | 3 | 1 | 6.76 | 32.94 |
F | 91 | - | 3 | - | 5 | 1 | 5.96 | 34.63 |
G | 95 | - | - | 3 | 1 | 1 | 7.10 | 34.75 |
H | 93 | - | - | 3 | 3 | 1 | 5.20 | 34.91 |
I | 91 | - | - | 3 | 5 | 1 | 3.95 | 34.95 |
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Hsueh, Y.-H.; Liaw, W.-C.; Kuo, J.-M.; Deng, C.-S.; Wu, C.-H. Hydrogel Film-Immobilized Lactobacillus brevis RK03 for γ-Aminobutyric Acid Production. Int. J. Mol. Sci. 2017, 18, 2324. https://doi.org/10.3390/ijms18112324
Hsueh Y-H, Liaw W-C, Kuo J-M, Deng C-S, Wu C-H. Hydrogel Film-Immobilized Lactobacillus brevis RK03 for γ-Aminobutyric Acid Production. International Journal of Molecular Sciences. 2017; 18(11):2324. https://doi.org/10.3390/ijms18112324
Chicago/Turabian StyleHsueh, Yi-Huang, Wen-Chang Liaw, Jen-Min Kuo, Chi-Shin Deng, and Chien-Hui Wu. 2017. "Hydrogel Film-Immobilized Lactobacillus brevis RK03 for γ-Aminobutyric Acid Production" International Journal of Molecular Sciences 18, no. 11: 2324. https://doi.org/10.3390/ijms18112324