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Int. J. Mol. Sci. 2017, 18(11), 2253;

Comparative Transcriptomic Analysis Reveals Candidate Genes and Pathways Involved in Larval Settlement of the Barnacle Megabalanus volcano

Department of Life Sciences, Institute of Deep-sea Science and Engineering, Chinese Academy of Sciences, Sanya 572000, China
College of Earth Sciences, University of Chinese Academy of Sciences, Beijing 100864, China
The Shenzhen Nobel Science and Technology Service Co., Ltd., Nanshan District, Shenzhen 440305, China
Division of Life Sciences, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
Author to whom correspondence should be addressed.
Received: 25 September 2017 / Revised: 14 October 2017 / Accepted: 23 October 2017 / Published: 27 October 2017
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Megabalanus barnacle is one of the model organisms for marine biofouling research. However, further elucidation of molecular mechanisms underlying larval settlement has been hindered due to the lack of genomic information thus far. In the present study, cDNA libraries were constructed for cyprids, the key stage for larval settlement, and adults of Megabalanus volcano. After high-throughput sequencing and de novo assembly, 42,620 unigenes were obtained with a N50 value of 1532 bp. These unigenes were annotated by blasting against the NCBI non-redundant (nr), Swiss-Prot, Cluster of Orthologous Groups (COG), and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Finally, 19,522, 15,691, 14,459, and 10,914 unigenes were identified correspondingly. There were 22,158 differentially expressed genes (DEGs) identified between two stages. Compared with the cyprid stage, 8241 unigenes were down-regulated and 13,917 unigenes were up-regulated at the adult stage. The neuroactive ligand-receptor interaction pathway (ko04080) was significantly enriched by KEGG enrichment analysis of the DEGs, suggesting that it possibly involved in larval settlement. Potential functions of three conserved allatostatin neuropeptide-receptor pairs and two light-sensitive opsin proteins were further characterized, indicating that they might regulate attachment and metamorphosis at cyprid stage. These results provided a deeper insight into the molecular mechanisms underlying larval settlement of barnacles. View Full-Text
Keywords: Megabalanus volcano; transcriptome; larval settlement; neuropeptide; opsin; GPCRs Megabalanus volcano; transcriptome; larval settlement; neuropeptide; opsin; GPCRs

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Yan, G.; Zhang, G.; Huang, J.; Lan, Y.; Sun, J.; Zeng, C.; Wang, Y.; Qian, P.-Y.; He, L. Comparative Transcriptomic Analysis Reveals Candidate Genes and Pathways Involved in Larval Settlement of the Barnacle Megabalanus volcano. Int. J. Mol. Sci. 2017, 18, 2253.

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