Collagen-Based Medical Device as a Stem Cell Carrier for Regenerative Medicine
AbstractMaintenance of mesenchymal stem cells (MSCs) requires a tissue-specific microenvironment (i.e., niche), which is poorly represented by the typical plastic substrate used for two-dimensional growth of MSCs in a tissue culture flask. The objective of this study was to address the potential use of collagen-based medical devices (HEMOCOLLAGENE®, Saint-Maur-des-Fossés, France) as mimetic niche for MSCs with the ability to preserve human MSC stemness in vitro. With a chemical composition similar to type I collagen, HEMOCOLLAGENE® foam presented a porous and interconnected structure (>90%) and a relative low elastic modulus of around 60 kPa. Biological studies revealed an apparently inert microenvironment of HEMOCOLLAGENE® foam, where 80% of cultured human MSCs remained viable, adopted a flattened morphology, and maintained their undifferentiated state with basal secretory activity. Thus, three-dimensional HEMOCOLLAGENE® foams present an in vitro model that mimics the MSC niche with the capacity to support viable and quiescent MSCs within a low stiffness collagen I scaffold simulating Wharton’s jelly. These results suggest that haemostatic foam may be a useful and versatile carrier for MSC transplantation for regenerative medicine applications. View Full-Text
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Aubert, L.; Dubus, M.; Rammal, H.; Bour, C.; Mongaret, C.; Boulagnon-Rombi, C.; Garnotel, R.; Schneider, C.; Rahouadj, R.; Laurent, C.; Gangloff, S.C.; Velard, F.; Mauprivez, C.; Kerdjoudj, H. Collagen-Based Medical Device as a Stem Cell Carrier for Regenerative Medicine. Int. J. Mol. Sci. 2017, 18, 2210.
Aubert L, Dubus M, Rammal H, Bour C, Mongaret C, Boulagnon-Rombi C, Garnotel R, Schneider C, Rahouadj R, Laurent C, Gangloff SC, Velard F, Mauprivez C, Kerdjoudj H. Collagen-Based Medical Device as a Stem Cell Carrier for Regenerative Medicine. International Journal of Molecular Sciences. 2017; 18(10):2210.Chicago/Turabian Style
Aubert, Léa; Dubus, Marie; Rammal, Hassan; Bour, Camille; Mongaret, Céline; Boulagnon-Rombi, Camille; Garnotel, Roselyne; Schneider, Céline; Rahouadj, Rachid; Laurent, Cedric; Gangloff, Sophie C.; Velard, Frédéric; Mauprivez, Cedric; Kerdjoudj, Halima. 2017. "Collagen-Based Medical Device as a Stem Cell Carrier for Regenerative Medicine." Int. J. Mol. Sci. 18, no. 10: 2210.
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