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Int. J. Mol. Sci. 2017, 18(10), 2210; https://doi.org/10.3390/ijms18102210

Collagen-Based Medical Device as a Stem Cell Carrier for Regenerative Medicine

1
Equipe d’Accueil 4691, Biomatériaux et Inflammation en Site Osseux (BIOS), Pôle Santé, UFR d’Odontologie, SFR-CAP Santé (FED 4231), Université de Reims Champagne-Ardenne, 1 Avenue du Maréchal Juin, 51100 Reims, France
2
UFR de Pharmacie, Université de Reims Champagne-Ardenne, 51100 Reims, France
3
UFR d’Odontologie, Université de Reims Champagne-Ardenne, 51100 Reims, France
4
Pole Pharmacie Pharmacovigilance CHU Reims, 51100 Reims, France
5
Laboratoire d’Anatomie et Cytologie Pathologiques, Centre Hospitalier-Universitaire, Hôpital Robert Debré, 51100 Reims, France
6
CNRS, UMR 7369, Medyc, Université de Reims Champagne-Ardenne, 51100 Reims, France
7
Equipe d’Accueil A 3795, Groupe d’Étude des Géomatériaux et Environnement Naturels, Anthropiques et Archéologiques (GEGENAA), Université de Reims Champagne Ardenne, 51100 Reims, France
8
CNRS, UMR 7563, LEMTA, Université de Lorraine, 54500 Vandœuvre-Lès-Nancy, France
These authors contributed equally to this work
*
Author to whom correspondence should be addressed.
Received: 26 September 2017 / Revised: 14 October 2017 / Accepted: 18 October 2017 / Published: 21 October 2017
(This article belongs to the Special Issue Novel Biomaterials for Tissue Engineering 2018)
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Abstract

Maintenance of mesenchymal stem cells (MSCs) requires a tissue-specific microenvironment (i.e., niche), which is poorly represented by the typical plastic substrate used for two-dimensional growth of MSCs in a tissue culture flask. The objective of this study was to address the potential use of collagen-based medical devices (HEMOCOLLAGENE®, Saint-Maur-des-Fossés, France) as mimetic niche for MSCs with the ability to preserve human MSC stemness in vitro. With a chemical composition similar to type I collagen, HEMOCOLLAGENE® foam presented a porous and interconnected structure (>90%) and a relative low elastic modulus of around 60 kPa. Biological studies revealed an apparently inert microenvironment of HEMOCOLLAGENE® foam, where 80% of cultured human MSCs remained viable, adopted a flattened morphology, and maintained their undifferentiated state with basal secretory activity. Thus, three-dimensional HEMOCOLLAGENE® foams present an in vitro model that mimics the MSC niche with the capacity to support viable and quiescent MSCs within a low stiffness collagen I scaffold simulating Wharton’s jelly. These results suggest that haemostatic foam may be a useful and versatile carrier for MSC transplantation for regenerative medicine applications. View Full-Text
Keywords: stem cell niche; medical device; regenerative medicine; biocompatibility; paracrine activities stem cell niche; medical device; regenerative medicine; biocompatibility; paracrine activities
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Aubert, L.; Dubus, M.; Rammal, H.; Bour, C.; Mongaret, C.; Boulagnon-Rombi, C.; Garnotel, R.; Schneider, C.; Rahouadj, R.; Laurent, C.; Gangloff, S.C.; Velard, F.; Mauprivez, C.; Kerdjoudj, H. Collagen-Based Medical Device as a Stem Cell Carrier for Regenerative Medicine. Int. J. Mol. Sci. 2017, 18, 2210.

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