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Int. J. Mol. Sci. 2017, 18(10), 2142; https://doi.org/10.3390/ijms18102142

Recent Advances in SELEX Technology and Aptamer Applications in Biomedicine

1,†
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2,3,4,5,†
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2,3,4,5,* , 2,3,4,5,* and 1,*
1
School of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong 999077, China
2
Law Sau Fai Institute for Advancing Translational Medicine in Bone & Joint Diseases, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong 999077, China
3
Institute of Integrated Bioinfomedicine and Translational Science, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong 999077, China
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Institute of Precision Medicine and Innovative Drug Discovery, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong 999077, China
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Shenzhen Lab of Combinatorial Compounds and Targeted Drug Delivery, HKBU Institute of Research and Continuing Education, Shenzhen 518000, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 20 September 2017 / Revised: 11 October 2017 / Accepted: 12 October 2017 / Published: 14 October 2017
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
View Full-Text   |   Download PDF [509 KB, uploaded 14 October 2017]   |  

Abstract

Aptamers are short DNA/RNA oligonucleotides capable of binding to target molecules with high affinity and specificity. The process of selecting an aptamer is called Systematic Evolution of Ligands by Exponential Enrichment (SELEX). Thanks to the inherit merits, aptamers have been used in a wide range of applications, including disease diagnosis, targeted delivery agents and therapeutic uses. To date, great achievements regarding the selection, modifications and application of aptamers have been made. However, few aptamer-based products have already successfully entered into clinical and industrial use. Besides, it is still a challenge to obtain aptamers with high affinity in a more efficient way. Thus, it is important to comprehensively review the current shortage and achievement of aptamer-related technology. In this review, we first present the limitations and notable advances of aptamer selection. Then, we compare the different methods used in the kinetic characterization of aptamers. We also discuss the impetus and developments of the clinical application of aptamers. View Full-Text
Keywords: aptamer; SELEX; selection; targeted drug delivery systems; therapy aptamer; SELEX; selection; targeted drug delivery systems; therapy
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Zhuo, Z.; Yu, Y.; Wang, M.; Li, J.; Zhang, Z.; Liu, J.; Wu, X.; Lu, A.; Zhang, G.; Zhang, B. Recent Advances in SELEX Technology and Aptamer Applications in Biomedicine. Int. J. Mol. Sci. 2017, 18, 2142.

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