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Int. J. Mol. Sci. 2017, 18(1), 206; doi:10.3390/ijms18010206

Reprogramming Methods Do Not Affect Gene Expression Profile of Human Induced Pluripotent Stem Cells

Department of Molecular Medicine, University of Padova, 35121 Padova, Italy
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Author to whom correspondence should be addressed.
Academic Editor: Anthony Lemarié
Received: 3 October 2016 / Revised: 29 December 2016 / Accepted: 13 January 2017 / Published: 20 January 2017
(This article belongs to the Special Issue Advances in Cell Transplantation)
View Full-Text   |   Download PDF [3669 KB, uploaded 20 January 2017]   |  

Abstract

Induced pluripotent stem cells (iPSCs) are pluripotent cells derived from adult somatic cells. After the pioneering work by Yamanaka, who first generated iPSCs by retroviral transduction of four reprogramming factors, several alternative methods to obtain iPSCs have been developed in order to increase the yield and safety of the process. However, the question remains open on whether the different reprogramming methods can influence the pluripotency features of the derived lines. In this study, three different strategies, based on retroviral vectors, episomal vectors, and Sendai virus vectors, were applied to derive iPSCs from human fibroblasts. The reprogramming efficiency of the methods based on episomal and Sendai virus vectors was higher than that of the retroviral vector-based approach. All human iPSC clones derived with the different methods showed the typical features of pluripotent stem cells, including the expression of alkaline phosphatase and stemness maker genes, and could give rise to the three germ layer derivatives upon embryoid bodies assay. Microarray analysis confirmed the presence of typical stem cell gene expression profiles in all iPSC clones and did not identify any significant difference among reprogramming methods. In conclusion, the use of different reprogramming methods is equivalent and does not affect gene expression profile of the derived human iPSCs. View Full-Text
Keywords: reprogramming method; induced pluripotent stem cells; retroviral vector; Sendai virus vector; episomal vector; gene expression reprogramming method; induced pluripotent stem cells; retroviral vector; Sendai virus vector; episomal vector; gene expression
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MDPI and ACS Style

Trevisan, M.; Desole, G.; Costanzi, G.; Lavezzo, E.; Palù, G.; Barzon, L. Reprogramming Methods Do Not Affect Gene Expression Profile of Human Induced Pluripotent Stem Cells. Int. J. Mol. Sci. 2017, 18, 206.

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Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
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