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Open AccessCommunication
Int. J. Mol. Sci. 2016, 17(9), 1532; doi:10.3390/ijms17091532

A Protocol to Enhance INS1E and MIN6 Functionality—The Use of Theophylline

1
Developmental BioEngineering, MIRA Institute of Biomedical Technology and Technical Medicine, University of Twente, Enschede 7522 NB, The Netherlands
2
Department of Complex Tissue Regeneration, MERLN Institute for Technology Inspired Regenerative Medicine, Maastricht University, Maastricht 6200 MD, The Netherlands
*
Author to whom correspondence should be addressed.
Academic Editor: Masatoshi Maki
Received: 1 August 2016 / Revised: 5 September 2016 / Accepted: 8 September 2016 / Published: 12 September 2016
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
View Full-Text   |   Download PDF [1247 KB, uploaded 12 September 2016]   |  

Abstract

In vitro research in the field of type I diabetes is frequently limited by the availability of a functional model for islets of Langerhans. This method shows that by the addition of theophylline to the glucose buffers, mouse insulinoma MIN6 and rat insulinoma INS1E pseudo-islets can serve as a model for islets of Langerhans for in vitro research. The effect of theophylline is dose- and cell line-dependent, resulting in a minimal stimulation index of five followed by a rapid return to baseline insulin secretion by reducing glucose concentrations after a first high glucose stimulation. This protocol solves issues concerning in vitro research for type I diabetes as donors and the availability of primary islets of Langerhans are limited. To avoid the limitations of using human donor material, cell lines represent a valid alternative. Many different β cell lines have been reported, but the lack of reproducible responsiveness to glucose stimulation remains a challenge. View Full-Text
Keywords: cell lines; insulin secretion in vitro; assay methods cell lines; insulin secretion in vitro; assay methods
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MDPI and ACS Style

Groot Nibbelink, M.; Marchioli, G.; Moroni, L.; Karperien, M.; Van Apeldoorn, A. A Protocol to Enhance INS1E and MIN6 Functionality—The Use of Theophylline. Int. J. Mol. Sci. 2016, 17, 1532.

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