Small GTPases and Stress Responses of vvran1 in the Straw Mushroom Volvariella volvacea
Abstract
:1. Introduction
2. Results
2.1. Identification of V. volvacea Small GTPases
2.2. Classification of V. volvacea Small GTPases
2.3. Phylogenic Analysis of VvRan1
2.4. The Superoxide Anion (O2−) Signal Molecular Triggers vvran1 to Respone Stresses
3. Discussion
4. Materials and Methods
4.1. Strains
4.2. Genome Sequencing, Splicing and Prediction
4.3. Annotation of Small GTPases
4.4. Validation of Sequence Accuracy
4.5. Sequence Homology Comparison and Phylogenetic Tree Construction
4.6. Preparation of Solutions and Stress Treatments
4.7. vvran1 Transcript Analysis
4.8. Histochemical Detection of O2−
4.9. Statistical Analysis
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Name | Gene ID_Predicted Method | GenBank ID | Name | Gene ID_Predicted Method | GenBank ID |
---|---|---|---|---|---|
VvArf1 | GME959_T | KU882137 | VvRab4 | GME5663_g | KU900094 |
VvArf2 | GME2124_T | KU882138 | VvRab5 | GME5664_g | KX009779 |
VvArf3 | GME4741_g | KU882139 | VvRab6 | GME7647_g | KU900095 |
VvArf4 | GME4841_g | KU882140 | VvRab7 | GME7649_g | KU900096 |
VvArf5 | GME5296_g | KU882141 | VvRab8 | GME7988_g | KU900097 |
VvArf6 | GME8402_T | KU882142 | VvRab9 | GME8752_g | KU900098 |
VvArf7 | GME8740_g | KU882143 | VvRab10 | GME9500_g | KU900099 |
VvArf8 | GME10621_g | KU882144 | VvRab11 | GME10910_g | KU900100 |
VvArf9 | GME10622_g | KU882145 | VvRab12 | GME11319_T | KU900101 |
VvRho1 | GME749_g | KU900090 | VvRab13 | GME11465_g | KU900102 |
VvRho2 | GME1391_g | KU900105 | VvRab14 | GME11526_g | KU900103 |
VvMitRho | GME1938_g | KU900106 | VvRab15 | GME11774_T | KU900104 |
VvRho4 | GME3984_G | KU900107 | VvRas1 | GME267_T | KX009781 |
VvRho5 | GME4319_g | KU900108 | VvRas2 | GME5033_g | KU900114 |
VvCdc42 | GME7713_T | KU900109 | VvRas3 | GME8078_g | KX009780 |
VvRho7 | GME7714_g | KU900110 | VvRas4 | GME8593_A | KU900118 |
VvRho8 | GME9067_g | KU900111 | VvRas5 | GME10486_g | KU900115 |
VvRho9 | GME9847_g | KU900112 | VvRas6 | GME11128_g | KU900116 |
VvRac | GME11424_T | KU900113 | VvRas7 | GME11133_T | KX009782 |
VvRab1 | GME3051_g | KU900091 | VvRas8 | GME11134_g | KU900117 |
VvRab2 | GME5340_g | KU900092 | VvRas9 | GME11562_T | AHA61595 |
VvRab3 | GME5662_g | KU900093 | VvRan1 | GME5409_T | KU882146 |
Species | Number of Proteins | ||||||
---|---|---|---|---|---|---|---|
Arf | Rho | Ras | Rab | Ran | Total | Reference Number | |
Schizophyllum commune | 7 | 7 | 5 | 14 | 1 | 34 | 34 a |
Laccaria bicolor | 9 | 21 | 12 | 10 | 1 | 53 | 55 b |
Pchrysosporium chrysosporium | 8 | 7 | 4 | 14 | 1 | 34 | 27 b |
Coprinopsis cinerea | 8 | 11 | 5 | 14 | 1 | 39 | 29 b |
Volvariella volvacea | 9 | 10 | 9 | 15 | 1 | 44 | This study |
Primer | Sequence (5′-3′) |
---|---|
Ran1-F | AGTTCGTCGCTGCTCCTGCTCT |
Ran1-R | ACCCTCAGCCTGTTCCAGTTCCTT |
GAPDH-F | CATCTTCCACTGGTGCGGCTAAG |
GAPDH-R | GGCTTCTCAAGGCGAACGACAA |
18S rRNA-F | TCTTGTGAAACTCTGTCGTGCTGGG |
18S rRNA-R | TTGCCCACACCCCAAAGCTAATTCG |
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Yan, J.-J.; Xie, B.; Zhang, L.; Li, S.-J.; Van Peer, A.F.; Wu, T.-J.; Chen, B.-Z.; Xie, B.-G. Small GTPases and Stress Responses of vvran1 in the Straw Mushroom Volvariella volvacea. Int. J. Mol. Sci. 2016, 17, 1527. https://doi.org/10.3390/ijms17091527
Yan J-J, Xie B, Zhang L, Li S-J, Van Peer AF, Wu T-J, Chen B-Z, Xie B-G. Small GTPases and Stress Responses of vvran1 in the Straw Mushroom Volvariella volvacea. International Journal of Molecular Sciences. 2016; 17(9):1527. https://doi.org/10.3390/ijms17091527
Chicago/Turabian StyleYan, Jun-Jie, Bin Xie, Lei Zhang, Shao-Jie Li, Arend F. Van Peer, Ta-Ju Wu, Bing-Zhi Chen, and Bao-Gui Xie. 2016. "Small GTPases and Stress Responses of vvran1 in the Straw Mushroom Volvariella volvacea" International Journal of Molecular Sciences 17, no. 9: 1527. https://doi.org/10.3390/ijms17091527