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Int. J. Mol. Sci. 2016, 17(9), 1442; doi:10.3390/ijms17091442

De Novo Analysis of the Transcriptome of Meloidogyne enterolobii to Uncover Potential Target Genes for Biological Control

1,2,†
,
1,†
,
3,4
,
1
and
1,*
1
Department of Plant Pathology, China Agricultural University, Beijing 100193, China
2
Beijing University of Agriculture, Beijing 102206, China
3
Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China
4
Hainan Engineering Technology Research Center for Tropical Ornamental Plant Germplasm Innovation and Utilization, Danzhou 571737, China
*
Author to whom correspondence should be addressed.
Academic Editor: Li Lin
Received: 23 June 2016 / Revised: 19 August 2016 / Accepted: 24 August 2016 / Published: 1 September 2016
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
View Full-Text   |   Download PDF [3252 KB, uploaded 1 September 2016]   |  

Abstract

Meloidogyne enterolobii is one of the obligate biotrophic root-knot nematodes that has the ability to reproduce on many economically-important crops. We carried out de novo sequencing of the transcriptome of M. enterolobii using Roche GS FLX and obtained 408,663 good quality reads that were assembled into 8193 contigs and 31,860 singletons. We compared the transcripts in different nematodes that were potential targets for biological control. These included the transcripts that putatively coded for CAZymes, kinases, neuropeptide genes and secretory proteins and those that were involved in the RNAi pathway and immune signaling. Typically, 75 non-membrane secretory proteins with signal peptides secreted from esophageal gland cells were identified as putative effectors, three of which were preliminarily examined using a PVX (pGR107)-based high-throughput transient plant expression system in Nicotiana benthamiana (N. benthamiana). Results showed that these candidate proteins suppressed the programmed cell death (PCD) triggered by the pro-apoptosis protein BAX, and one protein also caused necrosis, suggesting that they might suppress plant immune responses to promote pathogenicity. In conclusion, the current study provides comprehensive insight into the transcriptome of M. enterolobii for the first time and lays a foundation for further investigation and biological control strategies. View Full-Text
Keywords: Meloidogyne enterolobii; nematode; transcriptome; effector; pathogenicity Meloidogyne enterolobii; nematode; transcriptome; effector; pathogenicity
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Li, X.; Yang, D.; Niu, J.; Zhao, J.; Jian, H. De Novo Analysis of the Transcriptome of Meloidogyne enterolobii to Uncover Potential Target Genes for Biological Control. Int. J. Mol. Sci. 2016, 17, 1442.

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