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Int. J. Mol. Sci. 2016, 17(9), 1422; doi:10.3390/ijms17091422

Chemotactic Activity of Cyclophilin A in the Skin Mucus of Yellow Catfish (Pelteobagrus fulvidraco) and Its Active Site for Chemotaxis

1
College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China
2
Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China
3
Department of Zoology, Hazara University, Mansehra 21300, Pakistan
*
Authors to whom correspondence should be addressed.
Academic Editor: Helmut Segner
Received: 17 July 2016 / Revised: 16 August 2016 / Accepted: 23 August 2016 / Published: 29 August 2016
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
View Full-Text   |   Download PDF [1027 KB, uploaded 29 August 2016]   |  

Abstract

Fish skin mucus is a dynamic barrier for invading pathogens with a variety of anti-microbial enzymes, including cyclophilin A (CypA), a multi-functional protein with peptidyl-prolyl cis/trans isomerase (PPIase) activity. Beside various other immunological functions, CypA induces leucocytes migration in vitro in teleost. In the current study, we have discovered several novel immune-relevant proteins in yellow catfish skin mucus by mass spectrometry (MS). The CypA present among them was further detected by Western blot. Moreover, the CypA present in the skin mucus displayed strong chemotactic activity for yellow catfish leucocytes. Interestingly, asparagine (like arginine in mammals) at position 69 was the critical site in yellow catfish CypA involved in leucocyte attraction. These novel efforts do not only highlight the enzymatic texture of skin mucus, but signify CypA to be targeted for anti-inflammatory therapeutics. View Full-Text
Keywords: yellow catfish; skin mucus; cyclophilin A; chemotaxis; immune response yellow catfish; skin mucus; cyclophilin A; chemotaxis; immune response
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Dawar, F.U.; Tu, J.; Xiong, Y.; Lan, J.; Dong, X.X.; Liu, X.; Khattak, M.N.K.; Mei, J.; Lin, L. Chemotactic Activity of Cyclophilin A in the Skin Mucus of Yellow Catfish (Pelteobagrus fulvidraco) and Its Active Site for Chemotaxis. Int. J. Mol. Sci. 2016, 17, 1422.

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