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Int. J. Mol. Sci. 2016, 17(8), 1373; doi:10.3390/ijms17081373

Expression and Critical Role of Interleukin Enhancer Binding Factor 2 in Hepatocellular Carcinoma

1
Department of Hepatobiliary Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China
2
Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China
3
Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, Key Laboratory of Organ Transplantation, Hangzhou 310003, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Johannes Haybaeck
Received: 6 April 2016 / Revised: 5 August 2016 / Accepted: 8 August 2016 / Published: 22 August 2016
(This article belongs to the Collection Molecular Mechanisms of Human Liver Diseases)
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Abstract

Interleukin enhancer binding factor 2 (ILF2), a transcription factor, regulates cell growth by inhibiting the stabilization of mRNA. Currently, its role has gained recognition as a factor in the tumorigenic process. However, until now, little has been known about the detailed role ILF2 plays in hepatocellular carcinoma (HCC). In this study, we investigated the expression levels of ILF2 in HCC tissue with Western blot and immunohistochemical assays. To examine the effect of ILF2 on liver cancer cell growth and apoptosis, small interfering RNAs (siRNAs) targeting ILF2 were recombined to create lentiviral overexpression vectors. Our results showed higher expression levels of ILF2 mRNA and ILF2 protein in HCC tissue compared with matched peritumoral tissue. Expression of ILF2 may regulate cell growth and apoptosis in liver cancer cells via regulation of B-cell lymphoma 2 (Bcl-2), Bcl-2 related ovarian killer (Bok), Bcl-2-associated X protein (BAX), and cellular inhibitor of apoptosis 1 (cIAP1). Moreover, we inoculated nude mice with liver cancer cells to investigate the effect of ILF2 on tumorigenesis in vivo. As expected, a rapid growth was observed in cancer cells inoculated with a lentiviral vector coding Flag-ILF2 (Lenti-ILF2) compared with the control cells. Hence, these results promote a better understanding of ILF2’s potential role as a therapeutic target in HCC. View Full-Text
Keywords: interleukin enhancer binding factor 2; hepatocellular carcinoma; cell growth; apoptosis interleukin enhancer binding factor 2; hepatocellular carcinoma; cell growth; apoptosis
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Cheng, S.; Jiang, X.; Ding, C.; Du, C.; Owusu-Ansah, K.G.; Weng, X.; Hu, W.; Peng, C.; Lv, Z.; Tong, R.; Xiao, H.; Xie, H.; Zhou, L.; Wu, J.; Zheng, S. Expression and Critical Role of Interleukin Enhancer Binding Factor 2 in Hepatocellular Carcinoma. Int. J. Mol. Sci. 2016, 17, 1373.

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