Theophylline-Based KMUP-1 Improves Steatohepatitis via MMP-9/IL-10 and Lipolysis via HSL/p-HSL in Obese Mice
Abstract
:1. Introduction
2. Results
2.1. Body Weight Gain, sGOT, sGPT, Triglyceride (TG) and Glucose in Serum
2.2. Hematoxylin and Eosin (H&E) Staining of Livers
2.3. Immunohistochemistry (IHC) Staining of TNFα/MMP-9/HSL/p-HSL/ATGL in Steatohepatitis
2.4. IHC Staining of Type 1 or Type 2 Macrophages (M1 or M2) in Steatohepatitis
2.5. Expression of IL-10 and MMP-9 in HFD Livers
2.6. Effects of Hyperadiposity on Hepatic Reactive Oxygen Species (ROS)
3. Discussion
4. Materials and Methods
4.1. Animals and Blood Sampling
4.2. Measurement of Hepatic Oil Globules Diameter
4.3. Hematoxylin-Eosin (H&E) Staining of Liver Tissues
4.4. Immunohistochemistry (IHC) Staining of Liver Tissues and Macrophages
4.5. Western Blotting Analysis in Liver Tissues
4.6. Measurement of Hepatic ROS
4.7. Statistical Evaluation
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Wu, B.-N.; Kuo, K.-K.; Chen, Y.-H.; Chang, C.-T.; Huang, H.-T.; Chai, C.-Y.; Dai, Z.-K.; Chen, I.-J. Theophylline-Based KMUP-1 Improves Steatohepatitis via MMP-9/IL-10 and Lipolysis via HSL/p-HSL in Obese Mice. Int. J. Mol. Sci. 2016, 17, 1345. https://doi.org/10.3390/ijms17081345
Wu B-N, Kuo K-K, Chen Y-H, Chang C-T, Huang H-T, Chai C-Y, Dai Z-K, Chen I-J. Theophylline-Based KMUP-1 Improves Steatohepatitis via MMP-9/IL-10 and Lipolysis via HSL/p-HSL in Obese Mice. International Journal of Molecular Sciences. 2016; 17(8):1345. https://doi.org/10.3390/ijms17081345
Chicago/Turabian StyleWu, Bin-Nan, Kung-Kai Kuo, Yu-Hsun Chen, Chain-Ting Chang, Hung-Tu Huang, Chee-Yin Chai, Zen-Kong Dai, and Ing-Jun Chen. 2016. "Theophylline-Based KMUP-1 Improves Steatohepatitis via MMP-9/IL-10 and Lipolysis via HSL/p-HSL in Obese Mice" International Journal of Molecular Sciences 17, no. 8: 1345. https://doi.org/10.3390/ijms17081345