Next Article in Journal
Theophylline-Based KMUP-1 Improves Steatohepatitis via MMP-9/IL-10 and Lipolysis via HSL/p-HSL in Obese Mice
Previous Article in Journal
Chemokine-Like Receptor 1 mRNA Weakly Correlates with Non-Alcoholic Steatohepatitis Score in Male but Not Female Individuals
Article Menu
Issue 8 (August) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2016, 17(8), 1344; doi:10.3390/ijms17081344

Characterisation and Antioxidant Activity of Crude Extract and Polyphenolic Rich Fractions from C. incanus Leaves

1
Department of Agrifood Production and Environmental Sciences (DiSPAA), University of Florence, 50019 Sesto Fiorentino (Florence), Italy
2
Trees and Timber Institute (IVALSA), The National Research Council of Italy (CNR), 50019 Sesto Fiorentino (Florence), Italy
3
Institute for Plant Protection (IPSP), The National Research Council of Italy (CNR), 50019 Sesto Fiorentino (Florence), Italy
4
Department of Biotechnology, Chemistry and Pharmacy, University of Siena, 53100 Siena, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: David Arráez-Román
Received: 29 June 2016 / Revised: 5 August 2016 / Accepted: 10 August 2016 / Published: 17 August 2016
(This article belongs to the Section Bioactives and Nutraceuticals)
View Full-Text   |   Download PDF [1175 KB, uploaded 17 August 2016]   |  

Abstract

Cistus incanus (Cistaceae) is a Mediterranean evergreen shrub. Cistus incanus herbal teas have been used as a general remedy in traditional medicine since ancient times. Recent studies on the antioxidant properties of its aqueous extracts have indicated polyphenols to be the most active compounds. However, a whole chemical characterisation of polyphenolic compounds in leaves of Cistus incanus (C. incanus) is still lacking. Moreover, limited data is available on the contribution of different polyphenolic compounds towards the total antioxidant capacity of its extracts. The purpose of this study was to characterise the major polyphenolic compounds present in a crude ethanolic leaf extract (CEE) of C. incanus and develop a method for their fractionation. Superoxide anion, hydroxyl and DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging assays were also performed to evaluate the antioxidant properties of the obtained fractions. Three different polyphenolic enriched extracts, namely EAC (Ethyl Acetate Fraction), AF1 and AF2 (Aqueos Fractions), were obtained from CEE. Our results indicated that the EAC, enriched in flavonols, exhibited a higher antiradical activity compared to the tannin enriched fractions (AF1 and AF2). These findings provide new perspectives for the use of the EAC as a source of antioxidant compounds with potential uses in pharmaceutical preparations. View Full-Text
Keywords: polyphenolic enriched fractions; flavonols; LC–MS/MS (liquid chromatography–tandem Mass Spectrometry); DPPH radical-scavenging activity polyphenolic enriched fractions; flavonols; LC–MS/MS (liquid chromatography–tandem Mass Spectrometry); DPPH radical-scavenging activity
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Gori, A.; Ferrini, F.; Marzano, M.C.; Tattini, M.; Centritto, M.; Baratto, M.C.; Pogni, R.; Brunetti, C. Characterisation and Antioxidant Activity of Crude Extract and Polyphenolic Rich Fractions from C. incanus Leaves. Int. J. Mol. Sci. 2016, 17, 1344.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top