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Int. J. Mol. Sci. 2016, 17(9), 1397; doi:10.3390/ijms17091397

Calcium-Sensing Receptor in Human Peripheral Blood T Lymphocytes Is Involved in the AMI Onset and Progression through the NF-κB Signaling Pathway

1
Department of Clinical Laboratory, Harbin Medical University Cancer Hospital, Harbin 150086, China
2
Blood Transfusion Department, the First Affiliated Hospital of Harbin Medical University, Harbin 150086, China
3
Department of Cardiology, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Kathleen Van Craenenbroeck
Received: 7 July 2016 / Revised: 28 July 2016 / Accepted: 16 August 2016 / Published: 24 August 2016
(This article belongs to the Collection G Protein-Coupled Receptor Signaling and Regulation)
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Abstract

Acute myocardial infarction (AMI) is a condition triggered by an inflammatory process that seriously affects human health. Calcium-sensing receptor (CaSR) in T lymphocytes is involved during the inflammation reaction. However, the relationship between them is not very clear. In this study, we collected human peripheral blood T lymphocytes from patients with AMI and in different stages of percutaneous coronary intervention (PCI) (at the onset of AMI, the first day after PCI (PCI-1), PCI-3, and PCI-5) to study the CaSR and NF-κB pathway protein expression, cytokine release and T cell apoptosis. The results showed that the expressions of CaSR, P-p65, Caspase-12, and the secretions of Th-1 and Th-2 type cytokines were increased at the onset of AMI, especially on the PCI-1. Meanwhile, the apoptosis rate of CD3+, CD4+ and CD8+ T lymphocytes also increased. However, from PCI-3, all the indicators began to decline. In addition, we also found that positive CaSR small interfering RNA (siRNA) transfection in T lymphocytes and NF-κB pathway blocker Bay-11-7082 reversed the increased expressions of CaSR, P-p65, Caspase-12, reduced the secretions of Th-1 and Th-2 type cytokines, and decreased T lymphocytes apoptosis rate not only in the AMI patients but also in the normal controls. All of these results indicated that CaSR in the human peripheral blood T lymphocytes were involved in the AMI onset and progression, which probably was related to the NF-κB pathway. Our study demonstrated the relationship between AMI and CaSR, and will provide new effective prevention theory and new targets for drug treatment. View Full-Text
Keywords: calcium-sensing receptor; signaling pathway; lymphocyte; acute myocardial infarction; cytokine calcium-sensing receptor; signaling pathway; lymphocyte; acute myocardial infarction; cytokine
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Zeng, J.-Y.; Du, J.-J.; Pan, Y.; Wu, J.; Bi, H.-L.; Cui, B.-H.; Zhai, T.-Y.; Sun, Y.; Sun, Y.-H. Calcium-Sensing Receptor in Human Peripheral Blood T Lymphocytes Is Involved in the AMI Onset and Progression through the NF-κB Signaling Pathway. Int. J. Mol. Sci. 2016, 17, 1397.

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