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Int. J. Mol. Sci. 2016, 17(8), 1287; doi:10.3390/ijms17081287

β-Ketoacyl-acyl Carrier Protein Synthase I (KASI) Plays Crucial Roles in the Plant Growth and Fatty Acids Synthesis in Tobacco

1
Key Laboratory of Tropical Plant Resource and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Xuefu Road 88, Kunming 650223, China
2
University of Chinese Academy of Science, Beijing 100049, China
3
College of Life Sciences, Anhui Science and Technology University, Fengyang 233100, China
4
Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Lanhei Road 132, Kunming 650201, China
*
Author to whom correspondence should be addressed.
Academic Editor: Marcello Iriti
Received: 21 June 2016 / Revised: 29 July 2016 / Accepted: 1 August 2016 / Published: 8 August 2016
(This article belongs to the Section Molecular Botany)
View Full-Text   |   Download PDF [10518 KB, uploaded 8 August 2016]   |  

Abstract

Fatty acids serve many functions in plants, but the effects of some key genes involved in fatty acids biosynthesis on plants growth and development are not well understood yet. To understand the functions of 3-ketoacyl-acyl-carrier protein synthase I (KASI) in tobacco, we isolated two KASI homologs, which we have designated NtKASI-1 and NtKASI-2. Expression analysis showed that these two KASI genes were transcribed constitutively in all tissues examined. Over-expression of NtKASI-1 in tobacco changed the fatty acid content in leaves, whereas over-expressed lines of NtKASI-2 exhibited distinct phenotypic features such as slightly variegated leaves and reduction of the fatty acid content in leaves, similar to the silencing plants of NtKASI-1 gene. Interestingly, the silencing of NtKASI-2 gene had no discernibly altered phenotypes compared to wild type. The double silencing plants of these two genes enhanced the phenotypic changes during vegetative and reproductive growth compared to wild type. These results uncovered that these two KASI genes had the partially functional redundancy, and that the KASI genes played a key role in regulating fatty acids synthesis and in mediating plant growth and development in tobacco. View Full-Text
Keywords: tobacco; 3-ketoacyl-ACP synthase I; over-expression; gene silence; fatty acid synthesis tobacco; 3-ketoacyl-ACP synthase I; over-expression; gene silence; fatty acid synthesis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Yang, T.; Xu, R.; Chen, J.; Liu, A. β-Ketoacyl-acyl Carrier Protein Synthase I (KASI) Plays Crucial Roles in the Plant Growth and Fatty Acids Synthesis in Tobacco. Int. J. Mol. Sci. 2016, 17, 1287.

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