Dermal Lipogenesis Inhibits Adiponectin Production in Human Dermal Fibroblasts while Exogenous Adiponectin Administration Prevents against UVA-Induced Dermal Matrix Degradation in Human Skin
Abstract
:1. Introduction
2. Results
2.1. The Effect of ADM on Cell Viability of Hs68 Fibroblasts
2.2. ADM Stimulated Lipid Accumulation in Hs68 Fibroblasts
2.3. Expression of Adipogenesis Markers in ADM-Induced Hs68 Fibroblasts
2.4. Adiponectin Can Increase Cell Adhesion Strength of UVA-Irradiated Hs68 Fibroblasts
2.5. Adiponectin Can Increase Elastic Modulus of UVA-Irradiated Hs68 Fibroblasts
2.6. The Effect of Adiponectin on Gene and Protein Expression of UVA-Irradiated Human Dermal Fibroblasts
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Reagents
4.2. UVA Irradiation and Treatment
4.3. Cell Viability
4.4. Oil Red O Staining
4.5. Triglyceride Assay
4.6. Measurement of Cell Adhesion Strength
4.7. The Young’s Modulus Analysis
4.8. Quantitative Real-Time PCR
4.9. Immunofluorescence Staining
4.10. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
Appendix A
References
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Primer | Sequence (Forward) | Sequence (Reverse) |
---|---|---|
GADPH | CATGAGAAGTATGACAACAGCCT | AGTCCTTCCACGATACCAAAGT |
PPAR-γ | AGCCTGCGAAAGCCTTTTGGTG | GGCTTCACATTCAGCAAACCTGG |
LPL | GGACTTGGAGATGTGGACCA | TGCTGCTTCTTTTGGCTCTG |
FABP4 | AAAGTCAAGAGCACCATAACC | TTCAATGCGAACTTCAGTCC |
Adiponectin | TATCCCCAACATGCCCATTCG | TAGGCAAAGTAGTACAGCCCA |
EGFR | TGGCATCTTTAAGGGCTCCA | TGGCTAGTCGGTGTAAACGT |
Adipo-RI | ACACCCTTCTCTGAGCCTTC | GGTGTGAAAGTGGGCTGAAG |
Adipo-RII | TGGAGCCCATTTTAGAGGCA | CACCGACCTTCCCATACCTT |
MMP-1 | CTGGCCACAACTGCCAAATG | CTGTCCCTGAACAGCCCAGTACTT |
MMP-3 | ATTCCATGGAGCCAGGCTTTC | CATTTGGGTCAAACTCCAACTGTG |
COX-2 | GCCCTTCACGTTATTGCAGATG | ATATGTTCTCCTGCCTACTGGAA |
Collagen-I | AAAAGGAAGCTTGGTCCACT | GTGTGGAGAAAGGAGCAGAA |
Collagen-III | GAAGGGCAGGGAACAACTTG | TTTGGCATGGTTCTGGCTTC |
Elastin | GGCCATTCCTGGTGGAGTTCC | AACTGGCTTAAGAGGTTTGCCTCCA |
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Fang, C.-L.; Huang, L.-H.; Tsai, H.-Y.; Chang, H.-I. Dermal Lipogenesis Inhibits Adiponectin Production in Human Dermal Fibroblasts while Exogenous Adiponectin Administration Prevents against UVA-Induced Dermal Matrix Degradation in Human Skin. Int. J. Mol. Sci. 2016, 17, 1129. https://doi.org/10.3390/ijms17071129
Fang C-L, Huang L-H, Tsai H-Y, Chang H-I. Dermal Lipogenesis Inhibits Adiponectin Production in Human Dermal Fibroblasts while Exogenous Adiponectin Administration Prevents against UVA-Induced Dermal Matrix Degradation in Human Skin. International Journal of Molecular Sciences. 2016; 17(7):1129. https://doi.org/10.3390/ijms17071129
Chicago/Turabian StyleFang, Chien-Liang, Ling-Hung Huang, Hung-Yueh Tsai, and Hsin-I Chang. 2016. "Dermal Lipogenesis Inhibits Adiponectin Production in Human Dermal Fibroblasts while Exogenous Adiponectin Administration Prevents against UVA-Induced Dermal Matrix Degradation in Human Skin" International Journal of Molecular Sciences 17, no. 7: 1129. https://doi.org/10.3390/ijms17071129