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Int. J. Mol. Sci. 2016, 17(7), 1095; doi:10.3390/ijms17071095

Regulation of Intestinal Epithelial Calcium Transport Proteins by Stanniocalcin-1 in Caco2 Cells

1,2,†
,
3,†
,
1,4,†
,
1
,
1
and
1,*
1
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, China
2
Department of Animal Science, Hubei Vocational College Of Bio-Technology, Wuhan 430070, Hubei, China
3
Hubei Key Laboratory of Embryo and Molecular Breeding, Hubei Academy of Agricultural Sciences, Wuhan 430064, Hubei, China
4
College of Animal Science, Yangtze University, Jingzhou 434023, Hubei, China
These authors contributed equally to this study.
*
Author to whom correspondence should be addressed.
Academic Editors: ChulHee Kang and Hiroyuki Kobori
Received: 14 April 2016 / Revised: 28 June 2016 / Accepted: 29 June 2016 / Published: 9 July 2016
(This article belongs to the Special Issue Calcium Regulation and Sensing)
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Abstract

Stanniocalcin-1 (STC1) is a calcium and phosphate regulatory hormone. However, the exact molecular mechanisms underlying how STC1 affects Ca2+ uptake remain unclear. Here, the expression levels of the calcium transport proteins involved in transcellular transport in Caco2 cells were examined following over-expression or inhibition of STC1. These proteins include the transient receptor potential vanilloid members (TRPV) 5 and 6, the plasma membrane calcium ATPase 1b (PMCA1b), the sodium/calcium exchanger (NCX1), and the vitamin D receptor (VDR). Both gene and protein expressions of TRPV5 and TRPV6 were attenuated in response to over-expression of STC1, and the opposite trend was observed in cells treated with siRNASTC1. To further investigate the ability of STC1 to influence TRPV6 expression, cells were treated with 100 ng/mL of recombinant human STC1 (rhSTC1) for 4 h following pre-transfection with siRNASTC1 for 48 h. Intriguingly, the increase in the expression of TRPV6 resulting from siRNASTC1 was reversed by rhSTC1. No significant effect of STC1 on the expression of PMCA1b, NCX1 or VDR was observed in this study. In conclusion, the effect of STC1 on calcium transport in intestinal epithelia is due to, at least in part, its negative regulation of the epithelial channels TRPV5/6 that mediate calcium influx. View Full-Text
Keywords: calcium; intestinal epithelium; stanniocalcin-1; TRPV5; TRPV6 calcium; intestinal epithelium; stanniocalcin-1; TRPV5; TRPV6
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Xiang, J.; Guo, R.; Wan, C.; Wu, L.; Yang, S.; Guo, D. Regulation of Intestinal Epithelial Calcium Transport Proteins by Stanniocalcin-1 in Caco2 Cells. Int. J. Mol. Sci. 2016, 17, 1095.

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