Transformation of Litchi Pericarp-Derived Condensed Tannin with Aspergillus awamori
AbstractCondensed tannin is a ubiquitous polyphenol in plants that possesses substantial antioxidant capacity. In this study, we have investigated the polyphenol extraction recovery and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity of the extracted polyphenol after litchi pericarp is treated with Aspergillus awamori, Aspergillus sojae or Aspergillus oryzae. We have further explored the activity of A. awamori in the formation of condensed tannin. The treatment of A. awamori appeared to produce the highest antioxidant activity of polyphenol from litchi pericarp. Further studies suggested that the treatment of A. awamori releases the non-extractable condensed tannin from cell walls of litchi pericarp. The total extractable tannin in the litchi pericarp residue after a six-time extraction with 60% ethanol increased from 199.92 ± 14.47–318.38 ± 7.59 μg/g dry weight (DW) after the treatment of A. awamori. The ESI-TOF-MS and HPLC-MS2 analyses further revealed that treatment of A. awamori degraded B-type condensed tannin (condensed flavan-3-ol via C4–C8 linkage), but exhibited a limited capacity to degrade the condensed tannin containing A-type linkage subunits (C4–C8 coupled C2–O–C7 linkage). These results suggest that the treatment of A. awamori can significantly improve the production of condensed tannin from litchi pericarp. View Full-Text
Share & Cite This Article
Lin, S.; Li, Q.; Yang, B.; Duan, X.; Zhang, M.; Shi, J.; Jiang, Y. Transformation of Litchi Pericarp-Derived Condensed Tannin with Aspergillus awamori. Int. J. Mol. Sci. 2016, 17, 1067.
Lin S, Li Q, Yang B, Duan X, Zhang M, Shi J, Jiang Y. Transformation of Litchi Pericarp-Derived Condensed Tannin with Aspergillus awamori. International Journal of Molecular Sciences. 2016; 17(7):1067.Chicago/Turabian Style
Lin, Sen; Li, Qing; Yang, Bao; Duan, Xuewu; Zhang, Mingwei; Shi, John; Jiang, Yueming. 2016. "Transformation of Litchi Pericarp-Derived Condensed Tannin with Aspergillus awamori." Int. J. Mol. Sci. 17, no. 7: 1067.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.