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Int. J. Mol. Sci. 2016, 17(7), 1063; doi:10.3390/ijms17071063

Digital Gene Expression Analysis of Ponkan Mandarin (Citrus reticulata Blanco) in Response to Asia Citrus Psyllid-Vectored Huanglongbing Infection

1
Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China
2
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
3
Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization Ministry of Agriculture, Guangzhou 510640, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Marcello Iriti
Received: 3 March 2016 / Revised: 16 June 2016 / Accepted: 27 June 2016 / Published: 2 July 2016
(This article belongs to the Special Issue Plant Innate Immunity)
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Abstract

Citrus Huanglongbing (HLB), the most destructive citrus disease, can be transmitted by psyllids and diseased budwoods. Although the final symptoms of the two main HLB transmission ways were similar and hard to distinguish, the host responses might be different. In this study, the global gene changes in leaves of ponkan (Citrus reticulata) mandarin trees following psyllid-transmission of HLB were analyzed at the early symptomatic stage (13 weeks post inoculation, wpi) and late symptomatic stage (26 wpi) using digital gene expression (DGE) profiling. At 13 wpi, 2452 genes were downregulated while only 604 genes were upregulated in HLB infected ponkan leaves but no pathway enrichment was identified. Gene function analysis showed impairment in defense at the early stage of infection. At late stage of 26 wpi, however, differentially expressed genes (DEGs) involved in carbohydrate metabolism, plant defense, hormone signaling, secondary metabolism, transcription regulation were overwhelmingly upregulated, indicating that the defense reactions were eventually activated. The results indicated that HLB bacterial infection significantly influenced ponkan gene expression, and a delayed response of the host to the fast growing bacteria might be responsible for its failure in fighting against the bacteria. View Full-Text
Keywords: Asian citrus psyllids (ACP); Citrus Huanglongbing (HLB); ponkan mandarin; differentially expressed genes (DEGs); digital gene expression (DGE) Asian citrus psyllids (ACP); Citrus Huanglongbing (HLB); ponkan mandarin; differentially expressed genes (DEGs); digital gene expression (DGE)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Zhong, Y.; Cheng, C.; Jiang, B.; Jiang, N.; Zhang, Y.; Hu, M.; Zhong, G. Digital Gene Expression Analysis of Ponkan Mandarin (Citrus reticulata Blanco) in Response to Asia Citrus Psyllid-Vectored Huanglongbing Infection. Int. J. Mol. Sci. 2016, 17, 1063.

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