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Int. J. Mol. Sci. 2016, 17(7), 1011; doi:10.3390/ijms17071011

Extraction, Characterization and Immunological Activity of Polysaccharides from Rhizoma gastrodiae

1
Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Sciences, Northwest A & F University, Yangling 712100, Shaanxi, China
2
College of Plant Protection, Northwest A & F University, Yangling 712100, Shaanxi, China
*
Author to whom correspondence should be addressed.
Academic Editor: Charles Brennan
Received: 12 May 2016 / Revised: 12 May 2016 / Accepted: 6 June 2016 / Published: 25 June 2016
(This article belongs to the Section Bioactives and Nutraceuticals)
View Full-Text   |   Download PDF [2041 KB, uploaded 25 June 2016]   |  

Abstract

A response surface and Box-Behnken design approach was applied to augment polysaccharide extraction from the residue of Rhizoma gastrodiae. Statistical analysis revealed that the linear and quadratic terms for three variables during extraction exhibited obvious effects on extraction yield. The optimum conditions were determined to be a liquid-to-solid ratio of 54 mL/g, an extraction temperature of 74 °C, an extraction time of 66 min, and three extractions. These conditions resulted in a maximum Rhizoma gastrodiae polysaccharide (RGP) extraction yield of 6.11% ± 0.13%. Two homogeneous polysaccharides (RGP-1a and RGP-1b) were obtained using DEAE cellulose-52 and Sephadex G-100 columns. The preliminary characterization of RGP-1a and RGP-1b was performed using HPLC-RID, HPGPC, and FTIR. Tests of the immunological activity in vitro showed that the two polysaccharides could significantly stimulate macrophages to release NO and enhance phagocytosis in a dose-dependent manner. In particular, RGP-1b (200 μg/mL) and LPS (2 μg/mL) had almost the same influence on the NO production and phagocytic activity of RAW 264.7 macrophages (p > 0.05). All the data obtained indicate that RGP-1a and RGP-1b have the potential to be developed as a health food. View Full-Text
Keywords: Rhizoma gastrodiae; polysaccharides; optimized extraction; characterization; immunological activity Rhizoma gastrodiae; polysaccharides; optimized extraction; characterization; immunological activity
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MDPI and ACS Style

Chen, J.; Tian, S.; Shu, X.; Du, H.; Li, N.; Wang, J. Extraction, Characterization and Immunological Activity of Polysaccharides from Rhizoma gastrodiae. Int. J. Mol. Sci. 2016, 17, 1011.

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