Functional Characterization of Soybean Glyma04g39610 as a Brassinosteroid Receptor Gene and Evolutionary Analysis of Soybean Brassinosteroid Receptors
Abstract
:1. Introduction
2. Results
2.1. Isolation of Glyma04g39610 (GmBRI1b)
2.2. Identification of Other BR Receptor Genes in Soybean
2.3. Transcript Levels of GmBRI1b and Other BR Receptor Genes in Soybean
2.4. Subcellular Localization of GmBRI1b
2.5. Functional Analysis of GmBRI1b in Arabidopsis
2.6. Ectopic Over-Expression of GmBRI1b Increased the Hypocotyl Length of the bri1-5 bak1-1D Mutant and Changed the Responses of the Wild Type and bri1-5 bak1-1D Mutant to Brassinazole
2.7. Over-Expression of GmBRI1b Altered the Expression Level of BR Biosynthesis-Related Genes in the bri1-5 bak1-1D Mutant
2.8. Structural Modeling of Soybean BR Receptors
2.9. Evolutionary Analysis of BR Receptors in Plants
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth
4.2. Extraction of Genomic DNA and RNA and Reverse Transcription of mRNA
4.3. RACE Cloning of GmBRI1b
4.4. Analysis of Expression Patterns of Soybean BR Receptor Genes
4.5. Over-Expression of GmBRI1b in Arabidopsis
4.6. Subcellular Localization of GmBRI1b
4.7. Phenotypic Analysis of Transgenic Arabidopsis
4.8. Determination of the Expression Levels of BR Biosynthesis-Related Genes in the Wild Type, the Mutant, and Their Corresponding over-Expression Lines
4.9. Determination of the GmBRI1b Structure
4.10. Alignment of BR Receptors
4.11. Structural Modeling of Soybean BR Receptors
4.12. Estimation of Selection and Substitution Rates
4.13. Data Analysis
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
AA | Amino acid |
BAK1 | BRI1 ASSOCIATED RECEPTOR KINASE 1 |
BIN2 | BRASSINOSTEROID INSENSITIVE2 |
BKI1 | BRI1 KINASE INHIBITOR 1 |
BR | Brassinosteroid |
BRI1 | BRASSINOSTEROID INSENSITIVE 1 |
Brz | Brassinazole |
BZR1 | BRASSINAZOLE-RESISTANT1 |
ID | Island domain |
ORF | Open reading frame |
qRT-PCR | Quantitative real-time PCR |
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Gene | Locus | EST | TM | SP | KD | Length (AA) | Localization | Int/Ext |
---|---|---|---|---|---|---|---|---|
GmBRI1a | Glyma06g15270 | Yes | 784..806 | 1..20 | 871..1143 | 1184 | Plas | 0/1 |
GmBRI1b | Glyma04g39610 | Yes | 787..809 | 1..22 | 874..1146 | 1187 | Plas | 0/1 |
GmBRL1a | Glyma04g12860 | Yes | 827..849 | 1..43 | 928..1202 | 1207 | Cyto | 0/1 |
GmBRL1b | Glyma06g47870 | Yes | 843..865 | No | 912..1186 | 1211 | Plas | 0/1 |
GmBRL2a | Glyma05g26771 | Yes | 752..774 | 1..32 | 756..1038 | 1053 | Nucl | 1/2 |
GmBRL2b | Glyma08g09750 | Yes | no | 1..29 | 837..1121 | 1136 | Plas | 0/1 |
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Peng, S.; Tao, P.; Xu, F.; Wu, A.; Huo, W.; Wang, J. Functional Characterization of Soybean Glyma04g39610 as a Brassinosteroid Receptor Gene and Evolutionary Analysis of Soybean Brassinosteroid Receptors. Int. J. Mol. Sci. 2016, 17, 897. https://doi.org/10.3390/ijms17060897
Peng S, Tao P, Xu F, Wu A, Huo W, Wang J. Functional Characterization of Soybean Glyma04g39610 as a Brassinosteroid Receptor Gene and Evolutionary Analysis of Soybean Brassinosteroid Receptors. International Journal of Molecular Sciences. 2016; 17(6):897. https://doi.org/10.3390/ijms17060897
Chicago/Turabian StylePeng, Suna, Ping Tao, Feng Xu, Aiping Wu, Weige Huo, and Jinxiang Wang. 2016. "Functional Characterization of Soybean Glyma04g39610 as a Brassinosteroid Receptor Gene and Evolutionary Analysis of Soybean Brassinosteroid Receptors" International Journal of Molecular Sciences 17, no. 6: 897. https://doi.org/10.3390/ijms17060897