Next Article in Journal
Gibberellic Acid: A Key Phytohormone for Spikelet Fertility in Rice Grain Production
Next Article in Special Issue
EIYMNVPV Motif is Essential for A1CF Nucleus Localization and A1CF (-8aa) Promotes Proliferation of MDA-MB-231 Cells via Up-Regulation of IL-6
Previous Article in Journal / Special Issue
Variability in Immunohistochemical Detection of Programmed Death Ligand 1 (PD-L1) in Cancer Tissue Types
Article Menu
Issue 5 (May) cover image

Export Article

Open AccessCommunication
Int. J. Mol. Sci. 2016, 17(5), 792; doi:10.3390/ijms17050792

A Novel Technique to Detect EGFR Mutations in Lung Cancer

1,2,†
,
3,†
,
4,†
,
1
,
2,* and 1,*
1
Department of Respiratory Medicine, the Second Hospital, Dalian Medical University, Dalian 116023, China
2
Key Laboratory of Laboratory Medicine, Ministry of Education, Zhejiang Provincial Key Laboratory of Medical Genetics, Wenzhou Medical University, Wenzhou 325035, China
3
Department of Thoracic Surgery, the Second Hospital of Dalian Medical University, Dalian 116023, China
4
Department of Urinary Surgery, the Second Hospital, Dalian Medical University, Dalian 116023, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: William Chi-shing Cho
Received: 30 March 2016 / Revised: 17 May 2016 / Accepted: 18 May 2016 / Published: 23 May 2016
View Full-Text   |   Download PDF [5870 KB, uploaded 23 May 2016]   |  

Abstract

Epidermal growth factor receptor (EGFR) gene mutations occur in multiple human cancers; therefore, the detection of EGFR mutations could lead to early cancer diagnosis. This study describes a novel EGFR mutation detection technique. Compared to direct DNA sequencing detection methods, this method is based on allele-specific amplification (ASA), recombinase polymerase amplification (RPA), peptide nucleic acid (PNA), and SYBR Green I (SYBR), referred to as the AS-RPA-PNA-SYBR (ARPS) system. The principle of this technique is based on three continuous steps: ASA or ASA combined with PNA to prevent non-target sequence amplification (even single nucleotide polymorphisms, SNPs), the rapid amplification advantage of RPA, and appropriate SYBR Green I detection (the samples harboring EGFR mutations show a green signal). Using this method, the EGFR 19Del(2) mutation was detected in 5 min, while the EGFR L858R mutation was detected in 10 min. In this study, the detection of EGFR mutations in clinical samples using the ARPS system was compatible with that determined by polymerase chain reaction (PCR) and DNA sequencing methods. Thus, this newly developed methodology that uses the ARPS system with appropriate primer sets is a rapid, reliable, and practical way to assess EGFR mutations in clinical samples. View Full-Text
Keywords: RPA; ASA; SYBR; EGFR mutation; novel methodology; point-of-care test (POCT) RPA; ASA; SYBR; EGFR mutation; novel methodology; point-of-care test (POCT)
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Liu, Y.; Lei, T.; Liu, Z.; Kuang, Y.; Lyu, J.; Wang, Q. A Novel Technique to Detect EGFR Mutations in Lung Cancer. Int. J. Mol. Sci. 2016, 17, 792.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top