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Int. J. Mol. Sci. 2016, 17(6), 811; doi:10.3390/ijms17060811

EIYMNVPV Motif is Essential for A1CF Nucleus Localization and A1CF (-8aa) Promotes Proliferation of MDA-MB-231 Cells via Up-Regulation of IL-6

1
The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China
2
Laboratory of Organ Fibrosis Prophylaxis and Treatment by Combine Traditional Chinese and Western Medicine, Research Center of Combine Traditional Chinese and Western Medicine, Affiliated Traditional Medicine Hospital of Sichuan Medical University, Luzhou 646000, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editors: William Chi-shing Cho and Sanjay K. Srivastava
Received: 5 January 2016 / Revised: 5 April 2016 / Accepted: 20 May 2016 / Published: 25 May 2016
(This article belongs to the Special Issue Molecular Classification of Human Cancer: Diagnosis and Treatment)
View Full-Text   |   Download PDF [6526 KB, uploaded 25 May 2016]   |  

Abstract

Apobec-1 complementation factor (A1CF) is a heterogeneous nuclear ribonuceloprotein (hnRNP) and mediates apolipoprotein-B mRNA editing. A1CF can promote the regeneration of the liver by post-transcriptionally stabilizing Interleukin-6 (IL-6) mRNA. It also contains two transcriptional variants-A1CF64 and A1CF65, distinguished by the appearance of a 24-nucleotide motif which contributes to the corresponding eight-amino acid motif of EIYMNVPV. For the first time, we demonstrated that the EIYMNVPV motif was essential for A1CF nucleus localization, A1CF deficient of the EIYMNVPV motif, A1CF (-8aa) showed cytoplasm distribution. More importantly, we found that A1CF (-8aa), but not its full-length counterpart, can promote proliferation of MDA-MB-231 cells accompanied with increased level of IL-6 mRNA. Furthermore, silencing of IL-6 attenuated A1CF (-8aa)-induced proliferation in MDA-MB-231 cells. In conclusion, notably, these findings suggest that A1CF (-8aa) promoted proliferation of MDA-MB-231 cells in vitro viewing IL-6 as a target. Thus, the EIYMNVPV motif could be developed as a potential target for basal-like breast cancer therapy. View Full-Text
Keywords: Apobec-1 complementation factor (A1CF); EIYMNVPV motif; Interleukin-6 (IL-6); nucleus localization; proliferation; MDA-MB-231 cells Apobec-1 complementation factor (A1CF); EIYMNVPV motif; Interleukin-6 (IL-6); nucleus localization; proliferation; MDA-MB-231 cells
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Zhou, L.; Hao, J.; Yuan, Y.; Peng, R.; Wang, H.; Ni, D.; Gu, Y.; Huang, L.; Mao, Z.; Lyu, Z.; Du, Y.; Liu, Z.; Li, Y.; Ju, P.; Long, Y.; Liu, J.; Zhou, Q. EIYMNVPV Motif is Essential for A1CF Nucleus Localization and A1CF (-8aa) Promotes Proliferation of MDA-MB-231 Cells via Up-Regulation of IL-6. Int. J. Mol. Sci. 2016, 17, 811.

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