Next Article in Journal
Mechanism of Mitochondrial Connexin43′s Protection of the Neurovascular Unit under Acute Cerebral Ischemia-Reperfusion Injury
Previous Article in Journal
SARS Coronavirus Papain-Like Protease Inhibits the TLR7 Signaling Pathway through Removing Lys63-Linked Polyubiquitination of TRAF3 and TRAF6
Article Menu
Issue 5 (May) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2016, 17(5), 682; doi:10.3390/ijms17050682

Functional and Activity Analysis of Cattle UCP3 Promoter with MRFs-Related Factors

1,2,3
,
1,2,* , 1,2
,
3
,
1
and
1,2
1
Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Guizhou University, Guiyang 550025, China
2
College of Animal Science, Guizhou University, Guiyang 550025, China
3
College of Life Science, Guizhou University, Guiyang 550025, China
*
Author to whom correspondence should be addressed.
Academic Editor: Constantinos Stathopoulos
Received: 27 March 2016 / Revised: 9 April 2016 / Accepted: 13 April 2016 / Published: 5 May 2016
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
View Full-Text   |   Download PDF [4213 KB, uploaded 5 May 2016]   |  

Abstract

Uncoupling protein 3 (UCP3) is mainly expressed in muscle. It plays an important role in muscle, but less research on the regulation of cattle UCP3 has been performed. In order to elucidate whether cattle UCP3 can be regulated by muscle-related factors, deletion of cattle UCP3 promoter was amplified and cloned into pGL3-basic, pGL3-promoter and PEGFP-N3 vector, respectively, then transfected into C2C12 myoblasts cells and UCP3 promoter activity was measured using the dual-Luciferase reporter assay system. The results showed that there is some negative-regulatory element from −620 to −433 bp, and there is some positive-regulatory element between −433 and −385 bp. The fragment (1.08 kb) of UCP3 promoter was cotransfected with muscle-related transcription factor myogenic regulatory factors (MRFs) and myocyte-specific enhancer factor 2A (MEF2A). We found that UCP3 promoter could be upregulated by Myf5, Myf6 and MyoD and downregulated by MyoG and MEF2A. View Full-Text
Keywords: uncoupling protein 3 (UCP3); promoter; transcriptional activity; MRFs family; myocyte-specific enhancer factor 2A (MEF2A) uncoupling protein 3 (UCP3); promoter; transcriptional activity; MRFs family; myocyte-specific enhancer factor 2A (MEF2A)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Chen, W.; Xu, H.; Chen, X.; Liu, Z.; Zhang, W.; Xia, D. Functional and Activity Analysis of Cattle UCP3 Promoter with MRFs-Related Factors. Int. J. Mol. Sci. 2016, 17, 682.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top